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B6db references: qwertok

type Journal Article
authors Schmidt, A.
title D-Cysteine desulfhydrase from spinach
journal Methods Enzymol
ui qwertok
year (1987)
volume 143
number 3
pages 449-53.
abstract D-Cysteine desulfhydrase was prepd. from homogenized spinach leaves by polymin P and (NH4)2SO4 pptn., gel chromatog., DEAE-cellulose chromatog., and mol. sieving on Biogel A-1.5M. A 110-fold purifn. with 34% yield was obtained. The purified enzyme had a specific activity of 5.7 mmol H2S formed/mg protein/h. It was specific for D-cysteine and was not inhibited by 2 mM Mg2+, Ca2+, K+, Na+, or Al3+. The enzyme was assayed by measuring the formation of H2S from D-cysteine (H2S was detd. by a reaction that forms methylene blue).
last changed 2004/02/13 18:59

B6db references