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B6db families: a
Description Alanine--glyoxylate aminotransferase (family a)
Notes The sequences included here are close homologs of alanine-glyoxylate aminotransferase 1 from rat liver organelles, which is identical to serine-glyoxylate aminotransferase ( and also to asparagine-oxo-acid aminotransferase ( [Noguchi & Fujiwara (1988) JBC 263, 182].

The enzymes from Drosophila and Aedes also show alanine-glyoxylate aminotransferase activity.

We have included in this family a number of bacterial sequences, as they seem very closely related to the metazoan ones. It must be remarked, however, that for none of these bacterial enzymes activity has been tested biochemically. Even for the Nostoc (Anabaena) enzyme, whose structure has been published, functional validation is lacking.

PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences 30
Sequences in seed alignment
ArchaeaBBD72610 (Sulfodiicoccus acidiphilus); WP_054838694 (Sulfolobus metallicus); NP_343929 (Sulfolobus solfataricus P2);
BacteriaAAF10920 (Deinococcus radiodurans); NP_840135 (Nitrosomonas europaea); NP_760338 (Vibrio vulnificus CMCP6); ZP_00055197 (Magnetospirillum magnetotacticum); NP_893037 (Prochlorococcus marinus ); ZP_00115395 (Synechococcus sp. WH 8102); AAK65472 (Sinorhizobium meliloti); NP_719867 (Shewanella oneidensis MR-1); BAB72961 (Nostoc sp. PCC 7120);
FungiNP_116623 (Saccharomyces cerevisiae); RPB14510 (Morchella conica CCBAS932); XP_016611500 (Spizellomyces punctatus DAOM BR117);
MetazoaT24910 (Caenorhabditis elegans); AAL29468 (Aedes aegypti); NP_511062 (Drosophila melanogaster); SPYA_FELCA (Felis silvestris catus); AAK26375 (Heterodera glycines); EAA05410 (Anopheles gambiae str. PEST); CAA71715 (Cavia porcellus); SPYA_RAT (Rattus norvegicus); SPYA_CALJA (Callithrix jacchus); CAC17015 (Xenopus laevis); SPYA_MOUSE (Mus musculus); SPYA_HUMAN (Homo sapiens); SPYA_RABIT (Oryctolagus cuniculus);
Other_EukaryaXP_024572774 (Plasmopara halstedii); BAW00393 (Acanthamoeba castellanii);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence SPYA_RAT
Domain interval 45-398
Catalytic site 231 K
 Wu D, Feng M1, Wang ZX, Qiao K, Tachibana H, Cheng XJ (2018) Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii Parasit Vectors 11 604.

 Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012) Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus Acta Crystallogr D Biol Crystallogr 68 763-72.

 Littlechild,J.A. (2011) Thermophilic archaeal enzymes and applications in biocatalysis Biochem Soc Trans 39 155-8.

 Han GW, Schwarzenbacher R, Page R et al. (2005) Crystal structure of an alanine-glyoxylate aminotransferase from Anabaena sp. at 1.70 A resolution reveals a noncovalently linked PLP cofactor. Proteins 58 971-75.

 Han, Q.; Fang, J.; Li, J. (2002) 3-Hydroxykynurenine transaminase identity with alanine glyoxylate transaminase. A probable detoxification protein in Aedes aegypti J Biol Chem 277 15781-7.

 Han, Q.; Li, J. (2002) Comparative characterization of Aedes 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase and Drosophila serine pyruvate aminotransferase FEBS Lett 527 199-204.

 Lumb, M. J.; Danpure, C. J. (2000) Functional synergism between the most common polymorphism in human alanine:glyoxylate aminotransferase and four of the most common disease- causing mutations J Biol Chem 275 36415-22.

 Oda, T.; Miyajima, H.; Suzuki, Y.; Ito, T.; Yokota, S.; Hoshino, M.; Ichiyama, A. (1989) Purification and characterization of the active serine: pyruvate aminotransferase of rat liver mitochondria expressed in Escherichia coli J Biochem (Tokyo) 106 460-7.

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last changed 2019/08/12 09:21

B6db families