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B6db families: 2.6.1.50

2.6.1.50
Activity 2.6.1.50
Description Glutamine--scyllo-inosose aminotransferase.
Notes The aminotransferase from B. circulans is involved in the biosynthesis of butirosin (a 2-deoxystreptamine-containing aminoglycoside antibiotic). It catalyses the transamination both of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of amino-dideoxy-scyllo-inosose to 2-deoxystreptamine.
PDB 2C7T;2C81;
PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences
8
Sequences in seed alignment
BacteriaAAR98547 (Micromonospora echinospora); BAC41204 (Bacillus circulans); CAA70012 (Streptomyces griseus); AAD28492 (Streptomyces spectabilis); CAE22472 (Streptomyces tenebrarius); GLDSA_STRFR (Streptomyces fradiae); GLDSA_STRKN (Streptomyces kanamyceticus); AAD45549 (Streptomyces netropsis);

DISPLAY: Fasta format, alignment, hmm, hmm_local


Reference sequence CAE22472
Domain interval 22-413
Catalytic site 202 K
 
References
 Jnawali HN, Subba B, Liou K, Sohng JK. (2009) Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr Biotechnol Lett 31 869-75.

 Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB. (2005) The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine Org Biomol Chem 3 1410-8.

 Kharel, M. K,; Subba, B.; Lee, H. C.; Liou, K.; Sohng J.K. (2005) Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius Bioorg Med Chem Lett 15 89-92.

 Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. (2005) Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 J Antibiot (Tokyo) 58 766-74.

 Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K. (2002) Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans J Antibiot (Tokyo) 55 707-714.

 Ahlert, J.; Distler, J.; Mansouri, K.; Piepersberg, W. (1997) Identification of stsC, the gene encoding the L-glutamine:scyllo- inosose aminotransferase from streptomycin-producing Streptomycetes Arch Microbiol 168 102-13.

Articles on 2.6.1.50
last changed 2009/06/30 19:37

B6db families