Activities | Families | Sequences | Fold types | References | Help
B6db families: 4.2.1.20

4.2.1.20
Activity 4.2.1.20
Description Tryptophan synthase.
Notes The family comprises only the beta subunits of bacterial, archaeal and plant enzymes. The alpha subunits are not PLP-dependent. In the case of fungal enzymes, which are fusion proteins (alpha+beta), the domain corresponding to the alpha subunit has been removed from the sequence.
PDB 1TTP;1UBS;1BKS;
PLP Fold Type II
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type II

Number of sequences
57
Sequences in seed alignment
ArchaeaTRPB_METJA (Methanococcus jannaschii); TRB2_ARCFU (Archaeoglobus fulgidus); TRPB_HALVO (Halobacterium volcanii); TRB1_AERPE (Aeropyrum pernix); TRB1_SULSO (Sulfolobus solfataricus); TRPB_PYRKO (Pyrococcus kodakaraensis); TRPB_METVO (Methanococcus voltae); TRB1_ARCFU (Archaeoglobus fulgidus); TRB1_PYRAB (Pyrococcus abyssi); TRPB_THEAC (Thermoplasma acidophilum); TRPB_PYRHO (Pyrococcus horikoshii); TRB2_METTH (Methanobacterium thermoautotrophicum); TRB2_PYRAB (Pyrococcus abyssi); TRB2_AERPE (Aeropyrum pernix); TRB1_METTH (Methanobacterium thermoautotrophicum);
BacteriaTRPB_BUCDN (Buchnera aphidicola (subsp. Diuraphis noxia)); TRPB_RHIET (Rhizobium etli); TRPB_STRCO (Streptomyces coelicolor); TRB2_THEMA (Thermotoga maritima); TRPB_SALTY (Salmonella typhimurium); TRPB_MYCLE (Mycobacterium leprae); TRB2_AQUAE (Aquifex aeolicus); TRPB_ECOLI (Escherichia coli); TRPB_BUCMH (Buchnera aphidicola (subsp. Melaphis rhois)); TRPB_BACSU (Bacillus subtilis); TRPB_MYCTU (Mycobacterium tuberculosis); TRPB_LACCA (Lactobacillus casei); TRPB_PSEPU (Pseudomonas putida); TRPB_BUCSC (Buchnera aphidicola (subsp. Schlechtendalia chinensis)); TRB1_AQUAE (Aquifex aeolicus); TRPB_ACICA (Acinetobacter calcoaceticus); TRPB_HAEIN (Haemophilus influenzae); TRPB_BACST (Bacillus stearothermophilus); TRPB_THETH (Thermus aquaticus (subsp. thermophilus)); TRPB_RHOSH (Rhodobacter sphaeroides); TRPB_BUCAP (Buchnera aphidicola (subsp. Schizaphis graminum)); TRPB_MYCIT (Mycobacterium intracellulare); TRPB_PASMU (Pasteurella multocida); TRPB_CAUCR (Caulobacter crescentus); TRPB_CORGL (Corynebacterium glutamicum); TRPB_HELPY (Helicobacter pylori); TRPB_SYNY3 (Synechocystis sp. (strain PCC 6803)); TRPB_LACLA (Lactococcus lactis (subsp. lactis)); TRPB_PSEAE (Pseudomonas aeruginosa); TRPB_VIBPA (Vibrio parahaemolyticus); TRB1_THEMA (Thermotoga maritima); TRPB_CHLTR (Chlamydia trachomatis);
FungiAAF91181 (Emericella nidulans); TRP_YEAST (Saccharomyces cerevisiae); TRP_COPCI (Coprinus cinereus); TRP_SCHPO (Schizosaccharomyces pombe); TRP_NEUCR (Neurospora crassa);
ViridiplantaeTRP2_ARATH (Arabidopsis thaliana); TRP1_ARATH (Arabidopsis thaliana); TRPB_CAMAC (Camptotheca acuminata); T04330 (Oryza sativa); TRP2_MAIZE (Zea mays);

DISPLAY: Fasta format, alignment, hmm, hmm_local


Reference sequence TRPB_ECOLI
Domain interval 49-377
Catalytic site 86 K
 
References
 Shen H, Yang Y, Wang F, Zhang Y, Ye N, Xu S, Wang H. (2009) Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis Acta Biochim Biophys Sin (Shanghai) 41 379-88.

 Fehlner-Gardiner C, Roshick C, Carlson JH, Hughes S, Belland RJ, Caldwell HD, McClarty G. (2002) Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase J Biol Chem 277 26893-903.

 Hettwer, S.; Sterner, R. (2002) A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role J Biol Chem 277 8194-201.

 Xie, G.; Forst, C.; Bonner, C.; Jensen, R.A. (2002) Significance of two distinct types of tryptophan synthase beta chain in Bacteria, Archaea and higher plants Genome Biol 3 R-0004.

 Tang, X.F.; Ezaki, S.; Atomi, H.; Imanaka, T. (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon Eur J Biochem 267 6369-77.

 Kisaka, H.; Kisaka, M.; Lee, H. Y.; Kameya, T. (1998) Isolation of a cDNA for tryptophan synthase beta from rice and studies of its expression in a 5-methyltryptophan-resistant mutant of rice Plant Mol Biol 38 875-8.

 Berlyn, M.B.; Last, R.L.; Fink, G.R. (1989) A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana Proc Natl Acad Sci U S A 86 4604-8.

 Crawford, I.P.; Eberly, L. (1989) DNA sequence of the tryptophan synthase genes of Pseudomonas putida Biochimie 71 521-31.

 Hyde, C. C.; Ahmed, S. A.; Padlan, E. A.; Miles, E. W.; Davies, D. R. (1988) Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium J Biol Chem 263 17857-71.

 Zalkin H, Yanofsky C. (1982) Yeast gene TRP5: structure, function, regulation J Biol Chem 257 1491-500.

 Crawford IP, Nichols BP, Yanofsky C. (1980) Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium J Mol Biol 142 489-502.

Articles on 4.2.1.20
last changed 2008/10/24 10:25

B6db families