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B6db families: 4.2.3.1

4.2.3.1
Activity 4.2.3.1
Description Threonine synthase.
Notes Threonine synthase sequences can be grouped into two quite distinct subgroups, that however have not been classified as separate families.
A first subgroup comprises enzymes from higher plants (which are long and S-adenosylmethionine-sensitive) but also enzymes from cyanobacteria, archaebacteria, and several gram-positive species.
The second subgroup includes enzymes from fungi and from proteobacteria.
PDB 1KL7;1E5X;1UIN;1UIM;
PLP Fold Type II
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type II

Number of sequences
50
Sequences in seed alignment
ArchaeaACL16873 (Candidatus Methanosphaerula palustris); ABN07586 (Methanocorpusculum labreanum Z); EET29520 (Methanocaldococcus vulcanius); YP_503069 (Methanospirillum hungatei); NP_070145 (Archaeoglobus fulgidus); AAM05023 (Methanosarcina acetivorans); YP_001046628 (Methanoculleus marisnigri);
BacteriaNP_952746 (Geobacter sulfurreducens); ZP_00134963 (Actinobacillus pleuropneumoniae); NP_422193 (Caulobacter crescentus); ZP_00019695 (Chloroflexus aurantiacus); ZP_00179456 (Crocosphaera watsonii); NP_299502 (Xylella fastidiosa); THRC_SERMA (Serratia marcescens); CAD77052 (Pirellula sp.); AAK02199 (Pasteurella multocida); NP_371853 (Staphylococcus aureus); AAD35631 (Thermotoga maritima); AAC06690 (Aquifex aeolicus); AAO77508 (Bacteroides thetaiotaomicron); ZP_00072628 (Trichodesmium erythraeum); NP_637168 (Xanthomonas campestris); THRC_BUCAI (Buchnera aphidicola); THRC_BACSU (Bacillus subtilis); AAS80465 (Thermus thermophilus); ZP_00118375 (Cytophaga hutchinsonii); THRC_METGL (Methylobacillus glycogenes); THRC_SYNY3 (Synechocystis sp. (strain PCC 6803)); ZP_05495826 (Clostridium papyrosolvens); THRC_HAEIN (Haemophilus influenzae); THRC_PSEAE (Pseudomonas aeruginosa); AAM73252 (Chlorobium tepidum); AAN50945 (Leptospira interrogans); THRC_MYCTU (Mycobacterium tuberculosis); AAM25739 (Thermoanaerobacter tengcongensis); THRC_ECOLI (Escherichia coli); NP_231992 (Vibrio cholerae); THRC_BACHD (Bacillus halodurans); NP_720550 (Streptococcus mutans); THRC_BACSP (Bacillus sp.); ZP_00109087 (Nostoc punctiforme); THRC_CORGL (Corynebacterium glutamicum);
FungiEAL01705 (Candida albicans); THRC_ASHGO (Ashbya gossypii); EDP41573 (Malassezia globosa); THRC_YEAST (Saccharomyces cerevisiae); CAY68881 (Pichia pastoris);
ViridiplantaeBAC10696.1 (Oryza sativa (japonica cultivar-group)); NP_194713.1 (Arabidopsis thaliana); THRC_SOLTU (Solanum tuberosum);

DISPLAY: Fasta format, alignment, hmm, hmm_local


Reference sequence THRC_ECOLI
Domain interval 77-377
Catalytic site 107 K
 
References
 Graham DE, Taylor SM, Wolf RZ, Namboori SC. (2009) Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase Biochem J 424 467-78.

 Covarrubias AS, Högbom M, Bergfors T, Carroll P, Mannerstedt K, Oscarson S, Parish T, Jones TA, Mowbray SL. (2008) Structural, biochemical, and in vivo investigations of the threonine synthase from Mycobacterium tuberculosis J Mol Biol 381 622-33.

 Tang DW, Li LF, Yu YM, Liu XY, Su XD, Zhao X, Liang YH. (2007) Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus mutans Protein Pept Lett 14 836-8.

 Omi, R.; Goto, M.; Miyahara, I.; Mizuguchi, H.; Hayashi, H.; Kagamiyama, H.; Hirotsu, K. (2003) Crystal structures of threonine synthase from Thermus thermophilus HB8: conformational change, substrate recognition, and mechanism J Biol Chem 278 46035-45.

 Garrido-Franco, M.; Ehlert, S.; Messerschmidt, A.; Marinkovic, S.; Huber, R.; Laber, B.; Bourenkov, G. P.; Clausen, T. (2002) Structure and function of threonine synthase from yeast J Biol Chem 277 12396-405.

 Curien, G.; Dumas, R.; Ravanel, S.; Douce, R. (1996) Characterization of an Arabidopsis thaliana cDNA encoding an S- adenosylmethionine-sensitive threonine synthase. Threonine synthase from higher plants FEBS Lett 390 85-90.

 Malumbres, M.; Mateos, L. M.; Guerrero, C.; Martin, J. F. (1995) Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes Folia Microbiol 40 595-606.

 Malumbres, M.; Mateos, L. M.; Lumbreras, M. A.; Guerrero, C.; Martin, J. F. (1994) Analysis and expression of the thrC gene of Brevibacterium lactofermentum and characterization of the encoded threonine synthase Appl Environ Microbiol 60 2209-19.

 Clepet, C.; Borne, F.; Krishnapillai, V.; Baird, C.; Patte, J. C.; Cami, B. (1992) Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes Mol Microbiol 6 3109-19.

 Parsot, C.; Cossart, P.; Saint-Girons, I.; Cohen, G. N. (1983) Nucleotide sequence of thrC and of the transcription termination region of the threonine operon in Escherichia coli K12 Nucleic Acids Res 11 7331-45.

Articles on 4.2.3.1
last changed 2008/03/11 18:43

B6db families