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B6db families: 5.1.1.1.a

5.1.1.1 a
Activity 5.1.1.1
Description Alanine racemase. (family a)
Notes A family that includes only bacterial enzymes, with the exception of two sequences from S. pombe.

The Salmonella enzyme (ALR1) has a monomeric structure.

Additional_domain AAD22403.1 300-700

PDB 1SFT;1RCQ;4FS9;
PLP Fold Type III
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type III

Number of sequences
40
Sequences in seed alignment
BacteriaALR_BACST (Bacillus stearothermophilus); ALR_MYCTU (Mycobacterium tuberculosis); ZP_00061927 (Clostridium thermocellum ); AAD22403 (Enterococcus gallinarum); ADW54426 (Pseudomonas putida); ALR_MYCAV (Mycobacterium avium); ALR2_ECOLI (Escherichia coli); AAM25327 (Thermoanaerobacter tengcongensis MB4); VATG_ENTFA (Enterococcus faecalis); 3HA1_B (Bacillus Anthracis); ALR_ANASP (Anabaena sp. / Nostoc sp.); ALR_STRCO (Streptomyces coelicolor); ALR_HELPY (Helicobacter pylori); AAF23014 (Aquifex pyrophilus); ALR_TREPA (Treponema pallidum); AF430807_9 (Enterococcus faecalis); ALR2_KLEAE (Klebsiella aerogenes); ALR_BACPS (Bacillus psychrosaccharolyticus); ALR1_ECOLI (Escherichia coli); ALR_LACPL (Lactobacillus plantarum); BAE19803 (Pseudomonas fluorescens); ALR_LACRE (Lactobacillus reuteri); ALR2_PSEAE (Pseudomonas aeruginosa); 3HA1_A (Bacillus Anthracis); ALR2_BACSU (Bacillus subtilis); ALR_SYNY3 (Synechocystis sp. (strain PCC 6803)); ALR_AQUAE (Aquifex aeolicus); AAK53980 (Enterococcus casseliflavus); ALR1_PSEAE (Pseudomonas aeruginosa); ALR_MYCSM (Mycobacterium smegmatis); ALR1_SALTY (Salmonella typhimurium); ALR_LISMO (Listeria monocytogenes); ALR_STRPN (Streptococcus pneumoniae); ALR2_SALTY (Salmonella typhimurium); ALR_HAEIN (Haemophilus influenzae); ALR1_CLOAB (Clostridium acetobutylicum); BAC56127 (Bifidobacterium bifidum);
FungiALR1_SCHPO (Schizosaccharomyces pombe); ALR2_SCHPO (Schizosaccharomyces pombe);
MetazoaBAH22617 (Marsupenaeus japonicus);

DISPLAY: Fasta format, alignment, hmm, hmm_local


Reference sequence ALR_BACST
Domain interval 7-230
Catalytic site 39 K
 
References
 Xue Z1, Hu Y, Xu S, Ohnishi K, Ma Y, Ju J, Zhao B. (2013) Characterization and preliminary mutation analysis of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4 Extremophiles 17 611-21.

 Wu HM, Kuan YC, Chu CH, Hsu WH, Wang WC. (2012) Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants. PLoS One. 7 .

 Couņago RM1, Davlieva M, Strych U, Hill RE, Krause KL. (2009) Biochemical and structural characterization of alanine racemase from Bacillus anthracis (Ames). BMC Struct Biol. .

 Yoshikawa N, Okada S, Abe H. (2009) Molecular Characterization of Alanine Racemase in the Kuruma Prawn Marsupenaeus japonicus J Biochem 145 249-58.

 Ju, J.; Yokoigawa, K.; Misono, H.; Ohnishi, K. (2005) Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of gene products expressed in Escherichia coli J Biosci Bioeng 100 409-417.

 Strych, U.; Penland, R. L.; Jimenez, M.; Krause, K. L.; Benedik, M. J. (2001) Characterization of the alanine racemases from two mycobacteria FEMS Microbiol Lett 196 93-8.

 Uo, T.; Yoshimura, T.; Tanaka, N.; Takegawa, K.; Esaki, N. (2001) Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase J Bacteriol 183 2226-33.

 Arias, C. A.; Weisner, J.; Blackburn, J. M.; Reynolds, P. E. (2000) Serine and alanine racemase activities of VanT: a protein necessary for vancomycin resistance in Enterococcus gallinarum BM4174 Microbiology 146 1727-34.

 Kim, S.S; Yu, Y.G. (2000) Molecular cloning of an eExtremely thermostable alanine racemase from Aquifex pyrophilus and enzymatic characterization of the expressed protein J Biochem Mol Biol 33 82-8.

 Strych, U.; Huang, H. C.; Krause, K. L.; Benedik, M. J. (2000) Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1 Curr Microbiol 41 290-4.

 Okubo, Y.; Yokoigawa, K.; Esaki, N.; Soda, K.; Kawai, H. (1999) Characterization of psychrophilic alanine racemase from Bacillus psychrosaccharolyticus Biochem Biophys Res Commun 256 333-40.

 Shaw, J.P.; Petsko, G.A.; Ringe, D. (1997) Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution. Biochemistry 36 1329-1342.

 Neidhart, D. J.; Distefano, M. D.; Tanizawa, K.; Soda, K.; Walsh, C. T.; Petsko, G. A. (1987) X-ray crystallographic studies of the alanine-specific racemase from Bacillus stearothermophilus. Overproduction, crystallization, and preliminary characterization J Biol Chem 262 15323-6.

 Inagaki, K.; Tanizawa, K.; Badet, B.; Walsh, C. T.; Tanaka, H.; Soda, K. (1986) Thermostable alanine racemase from Bacillus stearothermophilus: molecular cloning of the gene, enzyme purification, and characterization Biochemistry 25 3268-74.

Articles on 5.1.1.1.a
last changed 2014/03/18 13:54

B6db families