B6db families : 1_4_4

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1.4.4.2

Activity

1.4.4.2

Description

Glycine dehydrogenase (decarboxylating).

Notes

In some organisms, the protein has two distinct subunits. See for example:
gi|14591729|ref|NP_143817.1| glycine dehydrogenase subunit 1 [Pyrococcus horikoshii]
gi|14591728|ref|NP_143816.1| glycine dehydrogenase subunit 2 [Pyrococcus horikoshii].

The two subunits are structurally similar and appear to have arisen by gene duplication and subsequent divergence. Only the second subunit, corresponding to the C-terminal half of the multi-domain proteins in known to bind the PLP.

PDB

1WYU;

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type I

Number of sequences

Sequences in seed alignment

Archaea	NP_143816.1 (Pyrococcus horikoshii); NP_342409 (Sulfolobus solfataricus);
Bacteria	NP_951437 (Geobacter sulfurreducens); GCSP_ECOLI (Escherichia coli); GCSP_SYNY3 (Synechocystis sp. PCC 6803); AB0111 (Yersinia pestis); AAK73853 (Brucella abortus); NP_840694 (Nitrosomonas europaea); NP_422146 (Caulobacter crescentus); GCSB_THET2 (Thermus thermophilus); NP_461969 (Salmonella typhimurium); GCSP_MYCTU (Mycobacterium tuberculosis); GCS2_BACSU (Bacillus subtilis); D81821 (Neisseria meningitidis (serogroup A)); NP_867901 (Pirellula sp.); ZP_00162707 (Anabaena variabilis);
Fungi	EAK92665 (Candida albicans); GCSP_YEAST (Saccharomyces cerevisiae); GCSP_SCHPO (Schizosaccharomyces pombe);
Metazoa	GCSP_HUMAN (Homo sapiens); NP_649989 (Drosophila melanogaster); CAG08109 (Tetraodon nigroviridis); CAE59244 (Caenorhabditis briggsae); AAH42245 (Xenopus laevis); GCSP_CHICK (Gallus gallus);
Viridiplantae	GCSP_ARATH (Arabidopsis thaliana); GCSP_PEA (Pisum sativum); GCSA_FLAPR (Flaveria pringlei); GCSP_SOLTU (Solanum tuberosum);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

GCSP_CHICK

Domain interval

540-934

Catalytic site

738 K

References

Hasse D.; Mikkat S.; Thrun H.A.; Hagemann, M.; Bauwe, H. (2007) Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803. FEBS Lett 581 1297-301.

Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N. (2005) Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia EMBO J 24 1523-1536.

Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N. (2003) Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system from Thermus thermophilus HB8 Acta Crystallogr D Biol Crystallogr 59 554-7..

Turner, S.R.; Ireland, R.; Rawsthorne, S. (1992) Cloning and characterization of the P subunit of glycine decarboxylase from pea (Pisum sativum) J Biol Chem 267 5355-60..

Fujiwara, K.; Okamura-Ikeda, K.; Motokawa, Y. (1987) Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system Biochem Biophys Res Commun 149 621-7..

Articles on 1.4.4.2

last changed

2008/01/22 15:09