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B6db families:
Description 8-amino-7-oxononanoate synthase
Notes A family constructed based on validated bacterial and plant sequences.
The proteins in this family show a strict phylogenetic relationship to those in family (glycine acetyltransferase). Additionally, at least some of the validated enzymes in the family also show a glycine acetyltransferase activity.
PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences 37
Sequences in seed alignment
BacteriaBIOF_BACSH (Bacillus sphaericus); YP_482841 (Frankia sp. CcI3); NP_777891 (Buchnera aphidicola); WP_011195999 (Symbiobacterium thermophilum); CAG20713 (Photobacterium profundum); ZP_00090882 (Azotobacter vinelandii); CAE49913 (Corynebacterium diphtheriae); NP_625531 (Streptomyces coelicolor); YP_144848 (Thermus thermophilus HB8); ZP_00314057 (Clostridium thermocellum); ZP_00238882 (Bacillus cereus); WP_010880373 (Aquifex aeolicus); NP_797493 (Vibrio parahaemolyticus); ZP_02169770 (Bacillus selenitireducens MLS10); YP_528607 (Saccharophagus degradans); BIOF_ECOLI (Escherichia coli); NP_904050 (Chromobacterium violaceum); ZP_01088761 (Blastopirellula marina); BIOF_ERWHE (Erwinia herbicola); WP_008990014 (Galbibacter marinus); BAB07626 (Bacillus halodurans); YP_001019727 (Methylibium petroleiphilum PM1); CAE55406 (Mycobacterium tuberculosis H37Rv); WP_071635593 (Flavobacterium johnsoniae); AAQ00666 (Prochlorococcus marinus); BIOF_BACSU (Bacillus subtilis); ZP_01462606 (Stigmatella aurantiaca); BAB39461 (Kurthia sp.); WP_011231613 (Geobacillus kaustophilus); NP_682121 (Thermosynechococcus elongatus BP-1); SNV76674 (Riemerella anatipestifer); NP_455337 (Salmonella enterica); NP_404761 (Yersinia pestis);
ViridiplantaeEDQ65436 (Physcomitrella patens); NP_001044174 (Oryza sativa); CAO45009 (Vitis vinifera); AAY82238 (Arabidopsis thaliana);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence BIOF_ECOLI
Domain interval 39-380
Catalytic site 236 K
 Ren X, Wang X, Shi H, Zhang X, Chen Z, Malhi KK, Ding C, Yu S (2018) Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence Sci Rep 8 14645.

 Kubota T, Izumi Y. (2012) Detection and characterization of a thermophilic biotin biosynthetic enzyme, 7-keto-8-aminopelargonic acid synthase, from various thermophiles. Biosci Biotechnol Biochem 76 685-90.

 Kubota T, Shimono J, Kanameda C, Izumi Y. (2007) The first Thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase: Cloning and overexpression of the gene from an extreme thermophile, Thermus thermophilus, and characterization of its gene product Biosci Biotechnol Biochem 23 3033-3040.

 Bhor VM, Dev S, Vasanthakumar GR, Kumar P, Sinha S, Surolia A. (2006) Broad substrate stereospecificity of the Mycobacterium tuberculosis 7-keto-8-aminopelargonic acid synthase: Spectroscopic and kinetic studies J Biol Chem 281 25076-88.

 Pinon V, Ravanel S, Douce R, Alban C. (2005) Biotin synthesis in plants. The first committed step of the pathway is catalyzed by a cytosolic 7-keto-8-aminopelargonic acid synthase Plant Physiol 139 1666-76.

 Alexeev, D.; Alexeeva, M.; Baxter, R. L.; Campopiano, D. J.; Webster, S. P.; Sawyer, L. (1998) The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme J Mol Biol 284 401-19.

 Ploux, O.; Marquet, A. (1992) The 8-amino-7-oxopelargonate synthase from Bacillus sphaericus. Purification and preliminary characterization of the cloned enzyme overproduced in Escherichia coli Biochem J 283 327-31.

Articles on
last changed 2008/01/17 18:30

B6db families