B6db families : 2_5_1

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2.5.1.47

Activity

2.5.1.47

Description

Cysteine synthase.

Notes

A rather heterogeneous family, strictly related to cystathionine-beta-synthase (EC 4.2.1.22); the plant enzymes are also strictly related to other enzymes involved in secondary metabolism, e.g. cyanoalanine synthase.
Several bacteria contain two enzymes with this activity: O-acetylserine sulfhydrylase A (cysK) and O-acetylserine sulfhydrylase B (cysM). In contrast to O-acetylserine sulfhydrylase A, O-acetylserine sulfhydrylase B is preferentially used for cysteine biosynthesis during anaerobic growth and is able to utilize thiosulfate as a substrate.

PDB

1OAS;1FCJ;1O58;1D6S;

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type II

Number of sequences

Sequences in seed alignment

Archaea	AAG01804 (Methanosarcina thermophila); ZP_00149387 (Methanococcoides burtonii);
Bacteria	BAA88310 (Streptococcus suis); NP_249623 (Pseudomonas aeruginosa); NP_294513 (Deinococcus radiodurans ); SRPG_SYNP7 (Synechococcus sp. PCC 7942); NP_993950 (Yersinia pestis); AAO35907 (Clostridium tetani); CYSM_SALTY (Salmonella typhimurium); AAG01002 (Selenomonas ruminantium); CYSK_BACSU (Bacillus subtilis); CYSM_BACSU (Bacillus subtilis); D86692 (Lactococcus lactis); AAG28533 (Geobacillus stearothermophilus); CYSK_HAEIN (Haemophilus influenzae); AAS81978 (Thermus thermophilus); CYSK_MYCTU (Mycobacterium tuberculosis); ZP_00017205 (Chloroflexus aurantiacus); CYSK_SALTY (Salmonella typhimurium); CYSM_CAMJE (Campylobacter jejuni); NP_624004 (Thermoanaerobacter tengcongensis); NP_240954 (Bacillus halodurans); CYSK_ECOLI (Escherichia coli); CYSK_STAAM (Staphylococcus aureus); NP_228474 (Thermotoga maritima MSB8); CYSK_FLASP (Flavobacterium sp.); CYSK_SYNY3 (Synechocystis sp. (strain PCC 6803));
Fungi	BAC82550 (Penicillium chrysogenum); CYSK_EMENI (Emericella nidulans); CAD88569 (Eremothecium gossypii); NP_595332 (Schizosaccharomyces pombe); EAK82828 (Ustilago maydis);
Viridiplantae	CYK2_ORYSA (Oryza sativa); CYSK_SOLTU (Solanum tuberosum); CYSK_WHEAT (Triticum aestivum); CYSK_SPIOL (Spinacia oleracea); CYSL_ARATH (Arabidopsis thaliana); CYSK_CITLA (Citrullus lanatus); CYSK_MAIZE (Zea mays); CYSL_SPIOL (Spinacia oleracea); CYSK_ARATH (Arabidopsis thaliana); CYSL_SOLTU (Solanum tuberosum); CYSM_ARATH (Arabidopsis thaliana);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

CYSK_SALTY

Domain interval

7-300

Catalytic site

41 K

References

Claus, M.T.; Zocher, G.E.; Maier, T.H.; Schulz, G.E. (2005) Structure of the O-Acetylserine Sulfhydrylase Isoenzyme CysM from Escherichia coli Biochemistry 44 8620-6. .

Fujita, Y.; Takegawa, K. (2004) Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe Biosci Biotechnol Biochem 68 306-11..

Lithgow, J.K., Hayhurst, E.J.; Cohen, G.; Aharonowitz, Y.; Foster, S.J. (2004) Role of a cysteine synthase in Staphylococcus aureus J Bacteriol 186 1579-90..

Miller MD, Schwarzenbacher R, von Delft F, Abdubek P, Ambing E, et al. (2004) Crystal structure of a tandem cystathionine--synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 Å resolution Proteins 57 213 - 217.

Saavedra CP, Encinas MV, Araya MA, Perez JM, Tantalean JC, Fuentes DE, Calderon IL, Pichuantes SE, Vasquez CC. (2004) Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V Biochimie 86 481-5..

Omura H, Kuroda M, Kobayashi M, Shimizu S, Yoshida T, Nagasawa T. (2003) Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming beta-cyano-L-alanine J Biosci Bioeng 95 470-5.

Mizuno, Y.; Miyashita, Y.; Yamagata, S.; Iwama, T.; Akamatsu, T. (2002) Cysteine synthase of an extremely thermophilic bacterium, Thermus thermophilus HB8 Biosci Biotechnol Biochem 66 549-57..

Borup, B., Ferry, J.G. (2000) O-Acetylserine sulfhydrylase from Methanosarcina thermophila J Bacteriol 182 45-50.

Jost, R.; Berkowitz, O.; Wirtz, M.; Hopkins, L.; Hawkesford, M. J.; Hell, R. (2000) Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana Gene 253 237-47..

Burkhard, P.; Rao, G. S.; Hohenester, E.; Schnackerz, K. D.; Cook, P. F.; Jansonius, J. N. (1998) Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium J Mol Biol 283 121-33.

Muller, R.; Kuttler, E.; Lanz, C.; Drewke, C.; Schmidt, K. (1996) Isolation of a gene encoding cysteine synthase from Flavobacterium K3- 15 FEMS Microbiol Lett 136 305-8..

Rolland, N.; Droux, M.; Lebrun, M.; Douce, R. (1993) O-acetylserine(thiol)lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform Arch Biochem Biophys 300 213-22..

Owais, W.M.; Gharaibeh, R. (1990) Cloning of the E. coli O-acetylserine sulfhydrylase gene: ability of the clone to produce a mutagenic product from azide and O-acetylserine Mutat Res 245 151-5.

Articles on 2.5.1.47

last changed

2008/10/24 10:19