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B6db families: 2.5.1.47

2.5.1.47
Activity 2.5.1.47
Description Cysteine synthase
Notes A rather heterogeneous family, strictly related to cystathionine-beta-synthase (EC 4.2.1.22); the plant enzymes are also strictly related to other enzymes involved in secondary metabolism, e.g. cyanoalanine synthase.
Several bacteria contain two enzymes with this activity: O-acetylserine sulfhydrylase A (cysK) and O-acetylserine sulfhydrylase B (cysM). In contrast to O-acetylserine sulfhydrylase A, O-acetylserine sulfhydrylase B is preferentially used for cysteine biosynthesis during anaerobic growth and is able to utilize thiosulfate as a substrate.
PDB 1OAS;1FCJ;1O58;1D6S;2BHS;5JIS;
PLP Fold Type II
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type II

Number of sequences
51
Sequences in seed alignment
ArchaeaAAG01804 (Methanosarcina thermophila); ZP_00149387 (Methanococcoides burtonii);
BacteriaNP_240954 (Bacillus halodurans); CYSK_MYCTU (Mycobacterium tuberculosis); SRPG_SYNP7 (Synechococcus sp. PCC 7942); CYSK_STAAM (Staphylococcus aureus); CYSK_SALTY (Salmonella typhimurium); CYSK_HAEIN (Haemophilus influenzae); NP_228474 (Thermotoga maritima MSB8); NP_294513 (Deinococcus radiodurans ); ADR71221 (Lactobacillus casei); AAS81978 (Thermus thermophilus); CYSK_FLASP (Flavobacterium sp.); NP_624004 (Thermoanaerobacter tengcongensis); D86692 (Lactococcus lactis); CYSM_SALTY (Salmonella typhimurium); CYSK_SYNY3 (Synechocystis sp. (strain PCC 6803)); ZP_00017205 (Chloroflexus aurantiacus); CYSK_BACSU (Bacillus subtilis); BAA88310 (Streptococcus suis); NP_249623 (Pseudomonas aeruginosa); YP_002427202 (Acidithiobacillus ferrooxidans); AAG01002 (Selenomonas ruminantium); CYSM_BACSU (Bacillus subtilis); AAG28533 (Geobacillus stearothermophilus); WP_002964172 (Brucella abortus); NP_993950 (Yersinia pestis); CYSK_ECOLI (Escherichia coli); CYSM_CAMJE (Campylobacter jejuni); AAO35907 (Clostridium tetani);
FungiCDS00538 (Sporisorium scitamineum); BAC82550 (Penicillium chrysogenum); NP_595332 (Schizosaccharomyces pombe); EAK82828 (Ustilago maydis); CYSK_EMENI (Emericella nidulans); CAD88569 (Eremothecium gossypii);
Other_EukaryaBBF16918 (Acanthamoeba castellanii); BAA21916 (Entamoeba histolytica);
ViridiplantaeCYSK_SPIOL (Spinacia oleracea); CYSM_ARATH (Arabidopsis thaliana); CYSK_CITLA (Citrullus lanatus); CYSK_WHEAT (Triticum aestivum); CYK2_ORYSA (Oryza sativa); BBE37478 (Leucaena leucocephala); CYSK_ARATH (Arabidopsis thaliana); CYSL_SPIOL (Spinacia oleracea); CYSL_ARATH (Arabidopsis thaliana); CYSL_SOLTU (Solanum tuberosum); CYSK_SOLTU (Solanum tuberosum); AHG97874 (Leucaena leucocephala); CYSK_MAIZE (Zea mays);

DISPLAY: Fasta format, alignment, hmm, hmm_local


Reference sequence CYSK_SALTY
Domain interval 8-301
Catalytic site 42 K
 
References
 Md Harun-Ur-Rashid, Iwasaki H, Parveen S, Oogai S, Fukuta M, Hossain M, Anai T, Oku H (2018) Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala Appl Microbiol Biotechnol. 186 613-632.

 Wu D, Feng M1, Wang ZX, Qiao K, Tachibana H, Cheng XJ (2018) Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii Parasit Vectors 11 604.

 Dharavath S, Raj I, Gourinath S (2017) Structure-based mutational studies of O-acetylserine sulfhydrylase reveal the reason for the loss of cysteine synthase complex formation in Brucella abortus Biochem J 474 1221-1239.

 Yafuso JT1, Negi VS, Bingham JP, Borthakur D. (2014) An O-Acetylserine (thiol) Lyase from Leucaena leucocephala Is a Cysteine Synthase But Not a Mimosine Synthase Appl Microbiol Biotechnol. 173 1157-68.

 Bogicevic B, Berthoud H, Portmann R, Meile L, Irmler S. (2012) CysK from Lactobacillus casei encodes a protein with O-acetylserine sulfhydrylase and cysteine desulfurization activity. Appl Microbiol Biotechnol. 94 1209-20.

 Tahir J, Watanabe M, Jing HC, Hunter DA, Tohge T, Nunes-Nesi A, Brotman Y, Fernie AR, Hoefgen R, Dijkwel PP. (2012) Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis Plant J. 73 118-130.

 Zheng C, Nie L, Qian L, Wang Z, Liu G, Liu J. (2009) K30, H150, and H168 Are Essential Residues for Coordinating Pyridoxal 5'-Phosphate of O-Acetylserine Sulfhydrylase from Acidithiobacillus ferrooxidans Curr Microbiol 60 461-5.

 Claus, M.T.; Zocher, G.E.; Maier, T.H.; Schulz, G.E. (2005) Structure of the O-Acetylserine Sulfhydrylase Isoenzyme CysM from Escherichia coli Biochemistry 44 8620-6.

 Fujita, Y.; Takegawa, K. (2004) Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe Biosci Biotechnol Biochem 68 306-11.

 Lithgow, J.K., Hayhurst, E.J.; Cohen, G.; Aharonowitz, Y.; Foster, S.J. (2004) Role of a cysteine synthase in Staphylococcus aureus J Bacteriol 186 1579-90.

 Miller MD, Schwarzenbacher R, von Delft F, Abdubek P, Ambing E, et al. (2004) Crystal structure of a tandem cystathionine--synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 resolution Proteins 57 213 - 217.

 Saavedra CP, Encinas MV, Araya MA, Perez JM, Tantalean JC, Fuentes DE, Calderon IL, Pichuantes SE, Vasquez CC. (2004) Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V Biochimie 86 481-5.

 Omura H, Kuroda M, Kobayashi M, Shimizu S, Yoshida T, Nagasawa T. (2003) Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming beta-cyano-L-alanine J Biosci Bioeng 95 470-5.

 Mizuno, Y.; Miyashita, Y.; Yamagata, S.; Iwama, T.; Akamatsu, T. (2002) Cysteine synthase of an extremely thermophilic bacterium, Thermus thermophilus HB8 Biosci Biotechnol Biochem 66 549-57.

 Borup, B., Ferry, J.G. (2000) O-Acetylserine sulfhydrylase from Methanosarcina thermophila J Bacteriol 182 45-50.

 Jost, R.; Berkowitz, O.; Wirtz, M.; Hopkins, L.; Hawkesford, M. J.; Hell, R. (2000) Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana Gene 253 237-47.

 Burkhard, P.; Rao, G. S.; Hohenester, E.; Schnackerz, K. D.; Cook, P. F.; Jansonius, J. N. (1998) Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium J Mol Biol 283 121-33.

 Muller, R.; Kuttler, E.; Lanz, C.; Drewke, C.; Schmidt, K. (1996) Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15 FEMS Microbiol Lett 136 305-8.

 Rolland, N.; Droux, M.; Lebrun, M.; Douce, R. (1993) O-acetylserine(thiol)lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform Arch Biochem Biophys 300 213-22.

 Owais, W.M.; Gharaibeh, R. (1990) Cloning of the E. coli O-acetylserine sulfhydrylase gene: ability of the clone to produce a mutagenic product from azide and O-acetylserine Mutat Res 245 151-5.

Articles on 2.5.1.47
last changed 2017/08/07 12:05

B6db families