B6db families : 2_5_1

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2.5.1.49

Activity

2.5.1.49

Description

O-acetylhomoserine (thiol)-lyase

Notes

Family of enzymes occurring in bacteria, fungi and archaea. THe prototype is the T. thermophilus enzyme, whose structure has been solved.
The sequences are strictly related to those of methionine gamma-lyases (family 4.4.1.11).

PDB

2CTZ;4KAM;

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type I

Number of sequences

Sequences in seed alignment

Archaea	AAM06094 (Methanosarcina acetivorans str. C2A); WP_094228163 (Methanolobus psychrotolerans); WP_048122994 (Methanosarcina barkeri CM1);
Bacteria	ZP_00115203 (Synechococcus sp. WH 8102); ZP_00121486 (Bifidobacterium longum DJO10A); CAA71732 (Leptospira meyeri); WP_074567952 (Bacillus cereus); NP_623710 (Thermoanaerobacter tengcongensis); WP_049624723 (Geobacillus stearothermophilus); NP_531944 (Agrobacterium tumefaciens str. C58 (U. Washington)); NP_385354 (Sinorhizobium meliloti); ZP_00107219 (Nostoc punctiforme); ZP_00082400 (Geobacter metallireducens); ZP_00119048 (Cytophaga hutchinsonii); APU51341 (Clostridioides difficile); NP_464123 (Listeria monocytogenes EGD-e); NP_540083 (Brucella melitensis); WP_024621399 (Clostridioides mangenotii); ACC39643 (Mycobacterium marinum M); BAB68505 (Thermus thermophilus); WP_061859240 (Clostridium colicanis); NP_349387 (Clostridium acetobutylicum); NP_228690 (Thermotoga maritima); NP_661504 (Chlorobium tepidum TLS);
Fungi	MT17_YEAST (Saccharomyces cerevisiae); MT17_KLULA (Kluyveromyces lactis); CYSD_EMENI (Emericella nidulans); CAB99179 (Neurospora crassa); AAF01452 (Candida albicans); CYSD_SCHPO (Schizosaccharomyces pombe);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

BAB68505

Domain interval

4-421

Catalytic site

206 K

References

Kulikova VV, Revtovich SV, Bazhulina NP, Anufrieva NV, Kotlov MI, Koval VS, Morozova EA, Hayashi H, Belyi YF, Demidkina TV (2019) Identification of O-acetylhomoserine sulfhydrylase, a putative enzyme responsible for methionine biosynthesis in Clostridioides difficile: Gene cloning and biochemical characterizations IUBMB Life 71 1815-1823.

Allen KD, Miller DV, Rauch BJ, Perona JJ, White RH (2015) Homocysteine is biosynthesized from aspartate semialdehyde and hydrogen sulfide in methanogenic archaea Biochemistry 54 3129-32.

Omura H, Ikemoto M, Kobayashi M, Shimizu S, Yoshida T, Nagasawa T (2003) Purification, characterization and gene cloning of thermostable O-acetyl-L-homoserine sulfhydrylase forming gamma-cyano-alpha-aminobutyric acid J Biosci Bioeng 96 53-8.

Shimizu H, Yamagata S, Masui R, Inoue Y, Shibata T, Yokoyama S, Kuramitsu S, Iwama T. (2001) Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product Biochim Biophys Acta 1549 1-72.

Yamagata, S.; Ichioka, K.; Goto, K.; Mizuno, Y; Iwama, T. (2001) Occurrence of transsulfuration in synthesis of L-homocysteine in an extremely thermophilic bacterium, Thermus thermophilus HB8 J Bacteriol 183 2086-92.

Bourhy, P.; Martel, A.; Margarita, D.; Saint Girons, I.; Belfaiza, J. (1997) Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited J Bacteriol 179 4396-4398.

Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R. (1994) Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine.O-acetylhomoserine sulfhydrylase of the yeast Appl Microbiol Biotechnol 42 92-99.

Brzywczy, J.; Yamagata, S.; Paszewski, A. (1993) Comparative studies on O-acetylhomoserine sulfhydrylase: physiological role and characterization of the Aspergillus nidulans enzyme Acta Biochim Pol 40 421-8.

Articles on 2.5.1.49

last changed

2019/08/28 12:15