B6db families : 2_6_1

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2.6.1.51

Activity

2.6.1.51

Description

Serine--pyruvate aminotransferase.

Notes

The sequences included here are close homologs of the enzyme from rat liver organelles, which is identical to isoenzyme 1 of alanine-glyoxylate aminotransferase (2.6.1.44) and also to asparagine-oxo-acid aminotransferase (2.6.1.14) [Noguchi & Fujiwara (1988) JBC 263, 182].
The enzymes from Drosophila and Aedes also show alanine-glyoxylate aminotransferase activity.

We have included in this family a number of bacterial sequences, as they seem very closely related to the metazoan ones. It must be remarked, however, that for none of these bacterial enzymes activity has been tested biochemically. Even for the Nostoc (Anabaena) enzyme, whose structure has been published, functional validation is lacking.

PDB

1H0C;

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type I

Number of sequences

Sequences in seed alignment

Bacteria

NP_840135 (Nitrosomonas europaea); AAF10920 (Deinococcus radiodurans); NP_719867 (Shewanella oneidensis MR-1); NP_760338 (Vibrio vulnificus CMCP6); AAK65472 (Sinorhizobium meliloti); ZP_00115395 (Synechococcus sp. WH 8102); ZP_00055197 (Magnetospirillum magnetotacticum); NP_893037 (Prochlorococcus marinus ); BAB72961.1 (Nostoc sp. PCC 7120);

Metazoa

CAA71715.1 (Cavia porcellus); SPYA_RAT (Rattus norvegicus); SPYA_HUMAN (Homo sapiens); EAA05410.1 (Anopheles gambiae str. PEST); AAL29468 (Aedes aegypti); T24910 (Caenorhabditis elegans); SPYA_MOUSE (Mus musculus); CAC17015 (Xenopus laevis); AAK26375 (Heterodera glycines); NP_511062 (Drosophila melanogaster); SPYA_FELCA (Felis silvestris catus); SPYA_CALJA (Callithrix jacchus); SPYA_RABIT (Oryctolagus cuniculus);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

SPYA_RAT

Domain interval

45-398

Catalytic site

231 K

References

Han GW, Schwarzenbacher R, Page R et al. (2005) Crystal structure of an alanine-glyoxylate aminotransferase from Anabaena sp. at 1.70 A resolution reveals a noncovalently linked PLP cofactor. Proteins 58 971-75.

Han, Q.; Fang, J.; Li, J. (2002) 3-Hydroxykynurenine transaminase identity with alanine glyoxylate transaminase. A probable detoxification protein in Aedes aegypti J Biol Chem 277 15781-7..

Han, Q.; Li, J. (2002) Comparative characterization of Aedes 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase and Drosophila serine pyruvate aminotransferase FEBS Lett 527 199-204..

Oda, T.; Miyajima, H.; Suzuki, Y.; Ito, T.; Yokota, S.; Hoshino, M.; Ichiyama, A. (1989) Purification and characterization of the active serine: pyruvate aminotransferase of rat liver mitochondria expressed in Escherichia coli J Biochem (Tokyo) 106 460-7..

Articles on 2.6.1.51

last changed

2007/11/30 14:25