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B6db families: a
Description Cysteine desulfurase (family a)
Notes This family encompasses eukariotic (mitochondrial) and bacterial cysteine desulfurases belonging to the ISC and SUF systems (these systems, devoted to the biosynthesis of iron-sulfur centers also include scaffold proteins, which function as sulfur and iron acceptors, as well as a variable number and type of accessory components).
Homologs of these proteins occur in archaea, but they have not been included here because none of them has been functionally validated.

Proteins in this family are phylogenetically related to selenocysteine lyases (EC, and often show some selenocysteine lyase activity.

PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences
Sequences in seed alignment
BacteriaCAD31372 (Mesorhizobium loti); ACK80697 (Acidithiobacillus ferrooxidans ATCC 23270); ISCS_HELPJ (Helicobacter pylori J99); NIFS_ENTAG (Enterobacter agglomerans); ISCS_RUMFL (Ruminococcus flavefaciens); BAA16678 (Synechocystis sp.); NP_360367 (Rickettsia conorii); CAC44787 (Rhizobium etli); NIFS_KLEPN (Klebsiella pneumoniae); ISCS_VIBCH (Vibrio cholerae); NIFS_ANASP (Anabaena sp.); AAC33372 (Cyanothece sp. PCC 8801); WP_016789103 (Vibrio cyclitrophicus); ZP_00108499 (Nostoc punctiforme); NIFS_AZOVI (Azotobacter vinelandii); BAA10109 (Synechocystis spp.); NP_519140 (Ralstonia solanacearum); 1ECXA (Thermotoga maritima); AAZ29040 (Streptomyces lividans); ISCS_PASMU (Pasteurella multocida); ISCS_HAEIN (Haemophilus influenzae); ISCS_ECOLI (Escherichia coli K12); AF105225_10 (Gluconacetobacter diazotrophicus); ISCS_SALTY (Salmonella typhimurium);
FungiNFS1_SCHPO (Schizosaccharomyces pombe); NFS1_CANMA (Candida maltosa); NFS1_YEAST (Saccharomyces cerevisiae);
MetazoaAAI34975 (Danio rerio); CAG31698 (Gallus gallus); NFS1_MOUSE (Mus musculus); XP_002167506 (Hydra magnipapillata); NFS1_HUMAN (Homo sapiens); NFS1_DROME (Drosophila melanogaster);
Other_EukaryaAAK69173 (Trichomonas vaginalis); CAZ34969 (Schistosoma mansoni); AAK39427 (Giardia intestinalis); AAK69174 (Trichomonas vaginalis);
ViridiplantaeNP_001142293 (Zea mays); AAK00758 (Arabidopsis thaliana); EDP02160 (Chlamydomonas reinhardtii); EEE90813 (Populus trichocarpa);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence ISCS_ECOLI
Domain interval 5-369
Catalytic site 206 K
 Liu L, Jiang S, Xing M, Chen C, Lai C, Li N, Liu , Wu D3, Gao H, Hong L, Tan P, Chen S, Deng Z, Wu G, Wang L (2020) Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System mBio 11 e00488-20.

 Ruiz M, Bettache A, Janicki A, Vinella D, Zhang CC, Latifi A. (2010) The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress FEBS J 277 3715-25.

 Marelja Z, Stöcklein W, Nimtz M, Leimkühler S. (2008) A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis J Biol Chem 283 25178-85.

 You D, Wang L, Yao F, Zhou X, Deng Z. (2007) A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans Biochemistry 46 6126-33.

 Zeng J, Zhang Y, Liu Y, Zhang X, Xia L, Liu J, Qiu G (2007) Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans Biotechnol Lett 29 1983-90.

 Biederbick A, Stehling O, Rösser R, Niggemeyer B, Nakai Y, Elsässer HP, Lill R. (2006) Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation Mol Cell Biol 26 5675-87.

 Muhlenhoff, U.; Balk, J.; Richhardt, N.; Kaiser, J. T.; Sipos, K.; Kispal, G.; Lill, R. (2004) Functional characterisation of the eukaryotic cysteine desulfurase Nfs1p from S. cerevisiae J Biol Chem 279 36906-15.

 Nilsson, K.; Lundgren, H.K.; Hagervall, T.G.; Bjork, G.R. (2002) The cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar typhimurium J Bacteriol 184 6830-5.

 Kaiser, J.T.; Clausen, T.; Bourenkow, G.P.; Bartunik, H.D.; Steinbacher, S.; Huber, R. (2000) Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly J Mol Biol 297 451-64.

 Kato, S.; Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N. (2000) Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation Biosci Biotechnol Biochem 64 2412-9.

 Kirby, J.; Wright, F.; Flint, H.J. (1998) A cysteine desulphurase gene from the cellulolytic rumen anaerobe Ruminococcus flavefaciens Biochim Biophys Acta 1386 233-7.

 Flint, D. H. (1996) Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase J Biol Chem 271 16068-74.

 Zheng, L.; White, R.H.; Cash, V.L.; Jack, R.F.; Dean, D.R. (1993) Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis Proc Natl Acad Sci U S A 90 2754-8.

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last changed 2020/05/08 01:57

B6db families