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B6db families: b
Description Glutamate decarboxylase. (family b)
Notes This family includes sequences from bacteria, fungi and plants. Similar sequences also exist in archaea, but they were not included here because none of them has been functionally validated.

Bacterial glutamate decarboxylase (GAD) is a homohexameric enzyme of about 330 kDa, which plays an important role in the maintenance of cellular homeostasis in acid environments.
Plant GAD differs from the bacterial enzyme in having a C-terminal extension of 33 amino acids within which resides a calmodulin (CaM)-binding domain. In plants, the enzyme product, GABA, is generally considered a metabolite, mostly produced in response to stress, but some evidences exist that it may also function as a signal molecule.

PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences
Sequences in seed alignment
BacteriaCAE33151 (Bordetella bronchiseptica); AAK47878 (Mycobacterium tuberculosis); ZP_00031802 (Burkholderia fungorum); BAB81764 (Clostridium perfringens); CAE20649 (Prochlorococcus marinus); ZP_00037596 (Enterococcus faecium); AAL82718 (Edwardsiella tarda); DCEB_LISMO (Listeria monocytogenes); DCEA_ECOLI (Escherichia coli); NP_786643 (Lactobacillus plantarum ); AAZ95185 (Lactobacillus brevis); NP_440384.1 (Synechocystis sp. PCC 6803); DCE_LACLC (Lactococcus lactis (subsp. cremoris)); T36342 (Streptomyces coelicolor); ZP_00023967 (Ralstonia metallidurans); AAS41604 (Bacillus cereus); NP_963191 (Mycobacterium avium ); BAC72367 (Streptomyces avermitilis); AAO77677 (Bacteroides thetaiotaomicron);
FungiEAA62607 (Aspergillus nidulans); CAB91726 (Neurospora crassa); EAA54393 (Magnaporthe grisea ); BAA88152 (Aspergillus oryzae);
ViridiplantaeDCE_LYCES (Lycopersicon esculentum); AHC56662 (Malus domestica); DCE2_ARATH (Arabidopsis thaliana); AAC39483 (Nicotiana tabacum); AHC56660 (Malus domestica); ABZ88153 (Zea mays); DCE_PETHY (Petunia hybrida); AAP46640 (Hordeum vulgare); AHC56661 (Malus domestica); ABA18653 (Pinus pinaster); DCE1_ARATH (Arabidopsis thaliana);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence DCEA_ECOLI
Domain interval 33-383
Catalytic site 276 K
 Christopher P Trobacher, Adel Zarei, Jingyun Liu, Shawn M Clark, Gale G Bozzo, Barry J Shelp (2013) Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit BMC Plant Biol 13 .

 Characterization and developmental expression of a glutamate decarboxylase from maritime pine. (2010) Molina-Rueda JJ1, Pascual MB, Cánovas FM, Gallardo F. planta 232 1471-83.

 Yunlong Zhuang, Guijie Ren, Chunmei He, Xinye Li, Qingmei Meng, Chuanfu Zhu, Rongchun Wang, Juren Zhang (2010) Cloning and Characterization of a Maize cDNA Encoding Glutamate Decarboxylase Plant Molecular Biology Reporter 28 620-626.

 Park, K-B; Oh, S-H (2007) Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3 Bioresource Technology 98 312-319.

 Oh, S-H.; Choi W-G.; Lee I-T.;Yun S-J. (2005) Cloning and characterization of a rice cDNA encoding glutamate decarboxylase J Biochem Mol Biol. 38 595-601.

 Capitani, G.; De Biase, D.; Aurizi, C.; Gut, H.; Bossa, F.; Grutter, M.G. (2003) Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase EMBO J. 22 4027-37.

 Kato, Y.; Kato, Y.; Furukawa, K.; Hara, S. (2002) Cloning and nucleotide sequence of the glutamate decarboxylase-encoding gene gadA from Aspergillus oryzae Biosci Biotechnol Biochem 66 2600-5.

 Nomura, M.; Nakajima, I.; Fujita, Y.; Kobayashi, M.; Kimoto, H.; Suzuki, I.; Aso, H. (1999) Lactococcus lactis contains only one glutamate decarboxylase gene Microbiology 145 1375-80.

 Turano, F. J.; Fang, T. K. (1998) Characterization of two glutamate decarboxylase cDNA clones from Arabidopsis Plant Physiol 117 1411-21.

 De Biase, D.; Tramonti, A.; John, R. A.; Bossa, F. (1996) Isolation, overexpression, and biochemical characterization of the two isoforms of glutamic acid decarboxylase from Escherichia coli Protein Expr Purif 8 430-8.

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last changed 2009/06/30 14:19

B6db families