B6db families : 4_2_1

	Activities \| Families \| Sequences \| Fold types \| References \| Help

4.2.1.20

Activity

4.2.1.20

Description

Tryptophan synthase.

Notes

The family comprises only the beta subunits of bacterial, archaeal and plant enzymes. The alpha subunits are not PLP-dependent. In the case of fungal enzymes, which are fusion proteins (alpha+beta), the domain corresponding to the alpha subunit has been removed from the sequence.

PDB

1TTP;1UBS;1BKS;

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type II

Number of sequences

Sequences in seed alignment

Archaea	TRB2_METTH (Methanobacterium thermoautotrophicum); TRPB_HALVO (Halobacterium volcanii); TRB1_ARCFU (Archaeoglobus fulgidus); TRB1_AERPE (Aeropyrum pernix); TRB1_SULSO (Sulfolobus solfataricus); TRPB_PYRKO (Pyrococcus kodakaraensis); TRPB_METJA (Methanococcus jannaschii); TRB2_AERPE (Aeropyrum pernix); TRB1_PYRAB (Pyrococcus abyssi); TRB2_PYRAB (Pyrococcus abyssi); TRB2_ARCFU (Archaeoglobus fulgidus); TRPB_THEAC (Thermoplasma acidophilum); TRPB_PYRHO (Pyrococcus horikoshii); TRPB_METVO (Methanococcus voltae); TRB1_METTH (Methanobacterium thermoautotrophicum);
Bacteria	TRPB_LACCA (Lactobacillus casei); TRB2_THEMA (Thermotoga maritima); TRPB_ECOLI (Escherichia coli); TRPB_VIBPA (Vibrio parahaemolyticus); TRPB_BUCAP (Buchnera aphidicola (subsp. Schizaphis graminum)); TRB2_AQUAE (Aquifex aeolicus); TRPB_STRCO (Streptomyces coelicolor); TRPB_BUCMH (Buchnera aphidicola (subsp. Melaphis rhois)); TRPB_MYCLE (Mycobacterium leprae); TRB1_AQUAE (Aquifex aeolicus); TRPB_LACLA (Lactococcus lactis (subsp. lactis)); TRPB_CORGL (Corynebacterium glutamicum); TRPB_CHLTR (Chlamydia trachomatis); TRPB_PSEPU (Pseudomonas putida); TRPB_MYCTU (Mycobacterium tuberculosis); TRPB_BUCSC (Buchnera aphidicola (subsp. Schlechtendalia chinensis)); TRPB_MYCIT (Mycobacterium intracellulare); TRPB_RHOSH (Rhodobacter sphaeroides); TRPB_BUCDN (Buchnera aphidicola (subsp. Diuraphis noxia)); TRPB_SALTY (Salmonella typhimurium); TRPB_SYNY3 (Synechocystis sp. (strain PCC 6803)); TRPB_PSEAE (Pseudomonas aeruginosa); TRPB_THETH (Thermus aquaticus (subsp. thermophilus)); TRPB_ACICA (Acinetobacter calcoaceticus); TRPB_PASMU (Pasteurella multocida); TRPB_CAUCR (Caulobacter crescentus); TRPB_BACST (Bacillus stearothermophilus); TRPB_HELPY (Helicobacter pylori); TRPB_RHIET (Rhizobium etli); TRPB_BACSU (Bacillus subtilis); TRB1_THEMA (Thermotoga maritima); TRPB_HAEIN (Haemophilus influenzae);
Fungi	AAF91181 (Emericella nidulans); TRP_COPCI (Coprinus cinereus); TRP_SCHPO (Schizosaccharomyces pombe); TRP_YEAST (Saccharomyces cerevisiae); TRP_NEUCR (Neurospora crassa);
Viridiplantae	TRP1_ARATH (Arabidopsis thaliana); TRPB_CAMAC (Camptotheca acuminata); T04330 (Oryza sativa); TRP2_MAIZE (Zea mays); TRP2_ARATH (Arabidopsis thaliana);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

TRPB_ECOLI

Domain interval

49-377

Catalytic site

86 K

References

Fehlner-Gardiner C, Roshick C, Carlson JH, Hughes S, Belland RJ, Caldwell HD, McClarty G. (2002) Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase J Biol Chem 277 26893-903.

Hettwer, S.; Sterner, R. (2002) A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role J Biol Chem 277 8194-201..

Xie, G.; Forst, C.; Bonner, C.; Jensen, R.A. (2002) Significance of two distinct types of tryptophan synthase beta chain in Bacteria, Archaea and higher plants Genome Biol 3 R-0004.

Tang, X.F.; Ezaki, S.; Atomi, H.; Imanaka, T. (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon Eur J Biochem 267 6369-77. .

Kisaka, H.; Kisaka, M.; Lee, H. Y.; Kameya, T. (1998) Isolation of a cDNA for tryptophan synthase beta from rice and studies of its expression in a 5-methyltryptophan-resistant mutant of rice Plant Mol Biol 38 875-8..

Berlyn, M.B.; Last, R.L.; Fink, G.R. (1989) A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana Proc Natl Acad Sci U S A 86 4604-8..

Crawford, I.P.; Eberly, L. (1989) DNA sequence of the tryptophan synthase genes of Pseudomonas putida Biochimie 71 521-31..

Hyde, C. C.; Ahmed, S. A.; Padlan, E. A.; Miles, E. W.; Davies, D. R. (1988) Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium J Biol Chem 263 17857-71..

Zalkin H, Yanofsky C. (1982) Yeast gene TRP5: structure, function, regulation J Biol Chem 257 1491-500.

Crawford IP, Nichols BP, Yanofsky C. (1980) Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium J Mol Biol 142 489-502.

Articles on 4.2.1.20

last changed

2008/10/24 10:25