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B6db families: 4.2.1.20

4.2.1.20
Activity 4.2.1.20
Description Tryptophan synthase
Notes The family comprises only the beta subunits of bacterial, archaeal and plant enzymes. The alpha subunits are not PLP-dependent. In the case of fungal enzymes, which are fusion proteins (alpha+beta), the domain corresponding to the alpha subunit has been removed from the sequence.
PDB 1TTP;1UBS;1BKS;1WDW;
PLP Fold Type II
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type II

Reference sequence TRPB_ECOLI
Domain interval 49-377
Catalytic site 86 K
 
References
 Buller AR, Brinkmann-Chen S, Romney DK, Herger M, Murciano-Calles J, Arnold FH (2015) Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation Proc Natl Acad Sci U S A 112 14599-604.

 Mitsuya D, Tanaka S, Matsumura H, Urano N, Takano K, Ogasahara K, Takehira M, Yutani K, Ishida M. (2014) Strategy for cold adaptation of the tryptophan synthase α subunit from the psychrophile Shewanella frigidimarina K14-2: crystal structure and physicochemical properties J Biochem 155 73-82.

 Shen H, Yang Y, Wang F, Zhang Y, Ye N, Xu S, Wang H. (2009) Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis Acta Biochim Biophys Sin (Shanghai) 41 379-88.

 Ogasahara K1, Ishida M, Yutani K. (2003) Stimulated interaction between and subunits of tryptophan synthase from hyperthermophile enhances its thermal stability J Biol Chem 278 8922-8.

 Fehlner-Gardiner C, Roshick C, Carlson JH, Hughes S, Belland RJ, Caldwell HD, McClarty G. (2002) Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase J Biol Chem 277 26893-903.

 Xie, G.; Forst, C.; Bonner, C.; Jensen, R.A. (2002) Significance of two distinct types of tryptophan synthase beta chain in Bacteria, Archaea and higher plants Genome Biol 3 R-0004.

 Tang, X.F.; Ezaki, S.; Atomi, H.; Imanaka, T. (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon Eur J Biochem 267 6369-77.

 Kisaka, H.; Kisaka, M.; Lee, H. Y.; Kameya, T. (1998) Isolation of a cDNA for tryptophan synthase beta from rice and studies of its expression in a 5-methyltryptophan-resistant mutant of rice Plant Mol Biol 38 875-8.

 Berlyn, M.B.; Last, R.L.; Fink, G.R. (1989) A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana Proc Natl Acad Sci U S A 86 4604-8.

 Crawford, I.P.; Eberly, L. (1989) DNA sequence of the tryptophan synthase genes of Pseudomonas putida Biochimie 71 521-31.

 Hyde, C. C.; Ahmed, S. A.; Padlan, E. A.; Miles, E. W.; Davies, D. R. (1988) Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium J Biol Chem 263 17857-71.

 Zalkin H, Yanofsky C. (1982) Yeast gene TRP5: structure, function, regulation J Biol Chem 257 1491-500.

 Crawford IP, Nichols BP, Yanofsky C. (1980) Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium J Mol Biol 142 489-502.

Articles on 4.2.1.20
last changed 2018/01/31 10:44

B6db families