B6db families : 5_1_1_5

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5.1.1.5 a

Activity

5.1.1.5

Description

Lysine racemase (family a)

Notes

Bacterial enzymes, some of them with very broad substrate specificity, most closely related to alanine racemases (family 5.1.1.1a). The enzyme from P. taetrolens is periplasmic.

PDB

4DZA;4DYJ;4BEU;

PLP Fold Type

III

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type III

Number of sequences

Sequences in seed alignment

Bacteria

AAF94470 (Vibrio cholerae O1 biovar El Tor str. N16961); NP_745855 (Pseudomonas putida KT2440); AAZ83975 (Pseudomonas putida IFO 12996); CUA81636 (Gulbenkiania indica); ADW54426 (Pseudomonas putida); WP_054306286 (Serratia rubidaea); WP_081547599 (Chromobacterium haemolyticum); WP_058911353 (Erwinia teleogrylli); WP_071472890 (Oceanisphaera psychrotolerans); WP_037400840 (Serratia sp. Ag1); WP_057390355 (Pseudomonas aeruginosa); WP_064560103 (Buttiauxella brennerae); WP_048389473 (Pseudomonas helleri); WP_004243720 (Proteus mirabilis); SDS16432 (Pseudomonas oryzae); ARGR_PSETA (Pseudomonas tetraolens); WP_070091016 (Pseudomonas monteilii); WP_027906812 (Pseudomonas taiwanensis); WP_032949360 (Citrobacter freundii); WP_066612162 (Crenobacter luteus);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

WP_004243720

Domain interval

47-406

Catalytic site

74 K

References

Espaillat A., Carrasco-López C, Bernardo-García N, Pietrosemoli N, Otero LH, Álvarez L, de Pedro MA, Pazos F, Davis BM, Waldor MK, Hermoso JA, Cava F. (2014) Structural basis for the broad specificity of a new family of amino-acid racemases. Acta Crystallogr D Biol Crystallogr. 70 79-90.

Radkov AD, Moe LA (2013) Amino acid racemization in Pseudomonas putida KT2440 J Bacteriol 195 5016-24.

Wu HM, Kuan YC, Chu CH, Hsu WH, Wang WC. (2012) Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants. PLoS One. 7 e48301.

Kuan YC, Kao CH, Chen CH, Chen CC, Hu HY, Hsu WH (2011) Biochemical characterization of a novel lysine racemase from Proteus mirabilis BCRC10725 Process Biochem 46 1914-20.

Matsui D, Oikawa T, Arakawa N, Osumi S, Lausberg F, Stäbler N, Freudl R, Eggeling L. (2009) A periplasmic, pyridoxal-5'-phosphate-dependent amino acid racemase in Pseudomonas taetrolens Appl Microbiol Biotechnol 83 1045-54.

Kino K, Sato M, Yoneyama M, Kirimura K. (2007) Synthesis of DL-tryptophan by modified broad specificity amino acid racemase from Pseudomonas putida IFO 12996 Appl Microbiol Biotechnol 73 1299-305.

Articles on 5.1.1.5.a

last changed

2018/04/17 08:54