B6db families : lipo

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lipo

Activity

Description

L-methionine--uridine-5'-aldehyde aminotransferase (2.6.1.-)

Notes

The enzymes in this family are related to 2_6_1_18 (beta-alanine-pyruvate transaminase), but the closest relative is family pumg (pseudouridine aldehyde aminotransferase) which in fact catalyzes a nearly identical reaction.
The enzymes from Streptomyces sp. SANK 60405 and Streptomyces roseoverticillatus (aka Streptoverticillium fervens) reportedly use L-methionine as the preferred amino group donor.
The enzyme from Streptomyces coeruleorubidus was tested only in the presence of L-alanine, but there is little doubt that it may work better with L-methionine, in analogy to the other two enzymes.

These enzymes are involved in the biogenesis of different antibiotics, such as the Uridyl peptide antibiotic pacidamycin. The pacidamycins belong to a class of antimicrobial nucleoside antibiotics that act by inhibiting translocase I, a key enzyme in peptidoglycan assembly.

PLP Fold Type

PLP-dependent Domain

Domain alignment
Domain hmm

Fold type I

Number of sequences

Sequences in seed alignment

Bacteria

WP_067307547 (Streptomyces griseochromogenes); ADC96664 (Streptomyces sp. SN-1061M); ADZ45332 (Streptomyces sp. NRRL 30471); ADN65338 (Streptomyces coeruleorubidus); BAO98809 (Streptomyces roseoverticillatus); AGG82467 (Streptomyces sp. SS); ACQ63626 (Streptomyces sp. MK730-62F2); BAJ05891 (Streptomyces sp. SANK 60405); SFY07327 (Streptomyces atratus);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence

BAJ05891

Domain interval

17-417

Catalytic site

271 K

References

Cui Z, Liu X, Overbay J, Cai W, Wang X, Lemke A, Wiegmann D, Niro G, Thorson JS, Ducho C, Van Lanen SG (2018) Enzymatic Synthesis of the Ribosylated Glycyl-Uridine Disaccharide Core of Peptidyl Nucleoside Antibiotics J Org Chem 83 7239-7249.

Hiratsuka T, Suzuki H, Kariya R, Seo T, Minami A, Oikawa H. (2014) Biosynthesis of the structurally unique polycyclopropanated polyketide-nucleoside hybrid jawsamycin (FR-900848). Angew Chem Int Ed Engl. 53 5423-6.

Chi X, Pahari P, Nonaka K, Van Lanen SG. (2011) Biosynthetic origin and mechanism of formation of the aminoribosyl moiety of peptidyl nucleoside antibiotics. J Am Chem Soc. 133 14452-9.

Ragab AE, Grüschow S, Tromans DR, Goss RJ (2011) Biogenesis of the unique 4',5'-dehydronucleoside of the uridyl peptide antibiotic pacidamycin J Am Chem Soc. 133 15288-91.

Articles on lipo

last changed

2018/05/21 12:20