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B6db families: lipo

lipo
Activity lipo
Description L-methionine--uridine-5'-aldehyde aminotransferase (2.6.1.-)
Notes The enzymes in this family are related to 2_6_1_18 (beta-alanine-pyruvate transaminase), but the closest relative is family pumg (pseudouridine aldehyde aminotransferase) which in fact catalyzes a nearly identical reaction.
The enzymes from Streptomyces sp. SANK 60405 and Streptomyces roseoverticillatus (aka Streptoverticillium fervens) reportedly use L-methionine as the preferred amino group donor.
The enzyme from Streptomyces coeruleorubidus was tested only in the presence of L-alanine, but there is little doubt that it may work better with L-methionine, in analogy to the other two enzymes.

These enzymes are involved in the biogenesis of different antibiotics, such as the Uridyl peptide antibiotic pacidamycin. The pacidamycins belong to a class of antimicrobial nucleoside antibiotics that act by inhibiting translocase I, a key enzyme in peptidoglycan assembly.

PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Reference sequence BAJ05891
Domain interval 17-417
Catalytic site 271 K
 
References
 Cui Z, Liu X, Overbay J, Cai W, Wang X, Lemke A, Wiegmann D, Niro G, Thorson JS, Ducho C, Van Lanen SG (2018) Enzymatic Synthesis of the Ribosylated Glycyl-Uridine Disaccharide Core of Peptidyl Nucleoside Antibiotics J Org Chem 83 7239-7249.

 Hiratsuka T, Suzuki H, Kariya R, Seo T, Minami A, Oikawa H. (2014) Biosynthesis of the structurally unique polycyclopropanated polyketide-nucleoside hybrid jawsamycin (FR-900848). Angew Chem Int Ed Engl. 53 5423-6.

 Chi X, Pahari P, Nonaka K, Van Lanen SG. (2011) Biosynthetic origin and mechanism of formation of the aminoribosyl moiety of peptidyl nucleoside antibiotics. J Am Chem Soc. 133 14452-9.

 Ragab AE, Grüschow S, Tromans DR, Goss RJ (2011) Biogenesis of the unique 4',5'-dehydronucleoside of the uridyl peptide antibiotic pacidamycin J Am Chem Soc. 133 15288-91.

Articles on lipo
last changed 2018/05/21 12:20

B6db families