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B6db families: lipo

Activity lipo
Description L-methionine--uridine-5'-aldehyde aminotransferase (2.6.1.-)
Notes The enzymes in this family are related to 2_6_1_18 (beta-alanine-pyruvate transaminase), but the closest relative is family pumg (pseudouridine aldehyde aminotransferase) which in fact catalyzes a nearly identical reaction.
The enzymes from Streptomyces sp. SANK 60405 and Streptomyces roseoverticillatus (aka Streptoverticillium fervens) reportedly use L-methionine as the preferred amino group donor.
The enzyme from Streptomyces coeruleorubidus was tested only in the presence of L-alanine, but there is little doubt that it may work better with L-methionine, in analogy to the other two enzymes.

These enzymes are involved in the biogenesis of different antibiotics, such as the Uridyl peptide antibiotic pacidamycin. The pacidamycins belong to a class of antimicrobial nucleoside antibiotics that act by inhibiting translocase I, a key enzyme in peptidoglycan assembly.

PLP Fold Type I
PLP-dependent Domain
Domain alignment
Domain hmm
Fold type I

Number of sequences 9
Sequences in seed alignment
BacteriaWP_067307547 (Streptomyces griseochromogenes); ADC96664 (Streptomyces sp. SN-1061M); ADZ45332 (Streptomyces sp. NRRL 30471); ADN65338 (Streptomyces coeruleorubidus); BAO98809 (Streptomyces roseoverticillatus); AGG82467 (Streptomyces sp. SS); ACQ63626 (Streptomyces sp. MK730-62F2); BAJ05891 (Streptomyces sp. SANK 60405); SFY07327 (Streptomyces atratus);

DISPLAY: Fasta format, alignment, hmm, hmm_local

Reference sequence BAJ05891
Domain interval 17-417
Catalytic site 271 K
 Cui Z, Liu X, Overbay J, Cai W, Wang X, Lemke A, Wiegmann D, Niro G, Thorson JS, Ducho C, Van Lanen SG (2018) Enzymatic Synthesis of the Ribosylated Glycyl-Uridine Disaccharide Core of Peptidyl Nucleoside Antibiotics J Org Chem 83 7239-7249.

 Hiratsuka T, Suzuki H, Kariya R, Seo T, Minami A, Oikawa H. (2014) Biosynthesis of the structurally unique polycyclopropanated polyketide-nucleoside hybrid jawsamycin (FR-900848). Angew Chem Int Ed Engl. 53 5423-6.

 Chi X, Pahari P, Nonaka K, Van Lanen SG. (2011) Biosynthetic origin and mechanism of formation of the aminoribosyl moiety of peptidyl nucleoside antibiotics. J Am Chem Soc. 133 14452-9.

 Ragab AE, Grüschow S, Tromans DR, Goss RJ (2011) Biogenesis of the unique 4',5'-dehydronucleoside of the uridyl peptide antibiotic pacidamycin J Am Chem Soc. 133 15288-91.

Articles on lipo
last changed 2018/05/21 12:20

B6db families