B6db activities : 2_6_1

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	2.6.1.109
Description	8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase
Alternative names	kdnA (gene name)
Catalyzed reaction	8-amino-3,8-dideoxy-alpha-D-manno-octulosonate + 2-oxoglutarate <=> 8-dehydro-3-deoxy-alpha-D-manno-octulosonate + L-glutamate
Cofactor	Pyridoxal-phosphate.
Comments	The enzyme, characterized from the bacterium Shewanella oneidensis, forms 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate, an aminated form of Kdo found in lipopolysaccharides of members of the Shewanella genus.
	Lipopolysaccharide (LPS) is composed of a hydrophobic anchor, known as lipid A, an inner core oligosaccharide, and a repeating O-antigen polysaccharide. In nearly all species, the first sugar bridging the hydrophobic lipid A and the polysaccharide domain is 3-deoxy-d-manno-octulosonic acid (Kdo), and thus it is critically important for LPS biosynthesis. Modifications to lipid A have been shown to be important for resistance to antimicrobial peptides as well as modulating recognition by the mammalian innate immune system. Therefore, lipid A derivatives have been used for development of vaccine strains and vaccine adjuvants. One derivative that has yet to be studied is 8-amino-3,8-dideoxy-d-manno-octulosonic acid (Kdo8N), which is found exclusively in marine bacteria of the genus Shewanella. Expression of these genes recombinantly in Escherichia coli resulted in lipid A containing Kdo8N, and in vitro assays confirmed their proposed enzymatic function.
PDB	5K8B;
Organisms	-Eubacteria
Family	2.6.1.109 (0)

Links	Enzyme (activities) 2.6.1.109 BRENDA (activities) 2.6.1.109 KEGG (pathways) 2.6.1.109 PLPMDB (PLP mutants) 2.6.1.109

References	Zachman-Brockmeyer TR, Thoden JB, Holden HM (2016) Structures of KdnB and KdnA from Shewanella oneidensis: Key Enzymes in the Formation of 8-Amino-3,8-Dideoxy-d-Manno-Octulosonic Acid Biochemistry 55 4485-94. Gattis SG, Chung HS, Trent MS, Raetz CR (2013) The origin of 8-amino-3,8-dideoxy-D-manno-octulosonic acid (Kdo8N) in the lipopolysaccharide of Shewanella oneidensis. J Biol Chem. 288 9216-25. Articles on 2.6.1.109

last changed	2017/09/06 11:31

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