type	Journal Article
authors	Tamura, K.; Nishiura, H.; Mori, J.; Imai, H.
title	Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana
journal	Biochem Soc Trans
Activity	2.3.1.50
Family	2.3.1.50.b
sel	selected
ui	11171191
year	(2000)
volume	28
number	6
pages	745-7
keywords	Acyltransferases/*genetics/*metabolism
abstract	The first and committed step in de novo sphingolipid synthesis is catalysed by serine palmitoyltransferase (EC 2.3.1.50), which condenses serine and palmitoyl-CoA to form 3-ketosphinganine in a pyridoxal-5'- phosphate-dependent reaction. We have isolated and characterized a cDNA clone from Arabidopsis thaliana that is homologous to yeast and mammalian LCB2. For a functional identification, the A. thaliana homologous cDNA was expressed in Escherichia coli, which resulted in significant production of new sphinganine in E. coli cells.
last changed	2009/01/12 19:20