type	Journal Article
authors	Li, J.; Shah, S.; Moffatt, B. A.; Glick, B. R.
title	Isolation and characterization of an unusual 1-aminocyclopropane-1- carboxylic acid (ACC) deaminase gene from Enterobacter cloacae UW4
journal	Antonie Van Leeuwenhoek
Activity	3.5.99.7
Family	3.5.99.7
sel	selected
ui	11827211
year	(2001)
volume	80
number	3-4
pages	255-61
keywords	Amino Acid Sequence
abstract	A genomic library of the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing plant growth-promoting bacterium Enterobacter cloacae UW4 in pUC19 in Escherichia coli was screened for the ability to utilize ACC as a sole source of nitrogen. One of the clones that was isolated contained a plasmid with an insert of approximately 0.8 kb that conferred ACC deaminase activity. Sequence analysis revealed that this DNA fragment contains an open-reading frame of 696 nucleotides predicted to encode a protein of 232 amino acids, a member of the amidohydrolase protein superfamily, i.e., a deaminase that contains a mononuclear or binuclear metal center as compared to the canonical ACC deaminase which contains pyridoxal phosphate as a co-factor.
last changed	2018/05/07 13:07