type	Journal Article
authors	Lucas, P.; Lonvaud-Funel, A.
title	Purification and partial gene sequence of the tyrosine decarboxylase of Lactobacillus brevis IOEB 9809
journal	FEMS Microbiol Lett
Activity	4.1.1.25
Family	4.1.1.25.a
sel	selected
ui	12052555
year	(2002)
volume	211
number	1
pages	85-9
abstract	Some lactic acid bacteria contain a tyrosine decarboxylase (TDC) which converts tyrosine to tyramine, a biogenic amine frequently encountered in fermented food and wine. Purification and microsequencing of the TDC of Lactobacillus brevis IOEB 9809 allowed us to determine a partial sequence of the TDC gene encoding 264 amino acids of the enzyme. Analysis of this protein sequence revealed typical features of pyridoxal phosphate-dependent amino acid decarboxylases while not any known decarboxylase was closely related to the TDC of L. brevis IOEB 9809. In addition, we could detect other L. brevis strains carrying a TDC gene in a rapid assay based on the polymerase chain reaction.
last changed	2009/06/12 11:27