type	Journal Article
authors	Tamegai, H.; Nango, E.; Kuwahara, M.; Yamamoto, H.; Ota, Y.; Kuriki, H.; Eguchi, T.; Kakinuma, K.
title	Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans
journal	J Antibiot (Tokyo)
Activity	2.6.1.101
sel	selected
ui	12374384.1
year	(2002)
volume	55
number	8
pages	707-714
abstract	Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.
last changed	2017/11/03 18:15