type	Journal Article
authors	Barajas JF, Zargar A, Pang B, Benites VT, Gin J, Baidoo EEK, Petzold CJ, Hillson NJ, Keasling JD
title	Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis
journal	Chembiochem
Activity	flvo
Family	flvo
sel	selected
ui	29603548
year	(2018)
volume	19
number	13
pages	1391-1395
keywords	Biosynthesis, Polyketide, Lactam, Fluvirucin, Transferase
abstract	Naturally occurring lactams, such as the polyketide-derived macrolactams, provide a diverse class of natural products that could enhance existing chemically produced lactams. While β-amino acid loading in the fluvirucin B2 polyketide pathway has been proposed by a previously identified putative biosynthetic gene cluster, biochemical characterization of the complete loading enzymes has not been described. Here we elucidate the complete biosynthetic pathway of the β-amino acid loading pathway in fluvirucin B2 biosynthesis. We demonstrate the promiscuity of the loading pathway to utilize a range of amino acids and further illustrate the ability to introduce non-native acyl transferases to selectively transfer β-amino acids onto a PKS loading platform. The results presented here provide a detailed biochemical description of β-amino acid selection and will further aid in future efforts to develop engineered lactam-producing PKS platforms.
last changed	2018/10/19 13:22