type	Journal Article
authors	Masada, M.; Fukushima, K.; Tamura, G.
title	Cysteine synthase from rape leaves
journal	J Biochem (Tokyo)
Activity	2.5.1.47
ui	76005462
year	(1975)
volume	77
number	5
pages	1107-15.
keywords	Chromatography, Gel
abstract	Cysteine synthase [O-Acetyl-L-serine acetate-lyase (adding hydrogen- sulfide) EC 4.2.99.8] has been highly purified from the extract of rape, Brassica chinensis var. Komatsuna. The purified preparation appeared to be homogeneous on Sephadex G-100 gel filtration and dodecylsulfate-polyacrylamide gel electrophoresis, showing a molecular weight of about 62,000. The latter method also suggested that this enzyme was composed of two identical subunits. The enzyme contained 2 moles of pyridoxal phosphate per mole of enzyme.
last changed	2003/03/17 14:53