type	Journal Article
authors	Meadows, G. G.; DiGiovanni, J.; Minor, L.; Elmer, G. W.
title	Some biological properties and an in vivo evaluation of tyrosine phenol- lyase on growth of B-16 melanoma
journal	Cancer Res
Activity	4.1.99.2
ui	76116118
year	(1976)
volume	36
number	1
pages	167-71.
keywords	Animal
abstract	Tyrosine phenol-lyase from Erwinia herbicola was purified with the goal of assessing its effect on growth of malignant melanoma. Ammonium sulfate-sodium citrate fractionation and diethylaminoethyl cellulose- hydroxylapatite chromatography were used. The purified enzyme was shown to reduce plasma tyrosine levels when administered to normal C57BL x DBA/2 F1 mice. The plasma half-life value of the enzyme was found to be 6 to 7 hr. Unlike results reported with glutaminase and asparaginase preparations, the lactate dehydrogenase-elevating virus had no significant influence on plasma clearance of tyrosine phenol-lyase. The enzyme significantly inhibited growth of established B-16 melanoma tumors.
last changed	2002/11/12 16:17