type	Journal Article
authors	Hodsdon, J.; Kolb, H.; Snell, E. E.; Cole, R. D.
title	The pyridoxal-binding site in pyridoxamine-pyruvate transaminase
journal	Biochem J
Activity	2.6.1.30
ui	78123679
year	(1978)
volume	169
number	2
pages	429-32.
keywords	Amino Acids/analysis
abstract	The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile-Tyr-Val-Thr-Gly- Pro-Asx-Lys(Pxy)-Cys-Leu(Pro2, Gly2, Ala2, Met)(Thr, Leu2)Gly-Val-Ser- Glu-Arg. This structure differs from those found for the corresponding peptides from pyridoxal phosphate-dependent enzymes.
last changed	2002/11/13 16:05