type	Journal Article
authors	Lam, V. M.; Chan, I. P.; Yeung, Y. G.
title	Role of L-threonine deaminase and L-threonine 3-dehydrogenase in the utilization of L-threonine by Pseudomonas aeruginosa
journal	J Gen Microbiol
Activity	4.3.1.19
Family	4.3.1.19
ui	81027443
year	(1980)
volume	117
number	2
pages	539-42.
keywords	Alcohol Oxidoreductases/*metabolism
abstract	Mutants of Pseudomonas aeruginosa PAC1 which could grow on L-threonine were isolated. These mutants, like the parent strain, synthesized a biosynthetic threonine deaminase, but its apparent Km value for threonine was higher than that of the enzyme from strain PAC1. These mutants also synthesized an inducible NAD-dependent threonine dehydrogenase, which was not present in the parent strain. No threonine aldolase activity could be detected. The results suggest that the threonine deaminase with lowered affinity for L-threonine, together with L-threonine dehydrogenase, enabled these mutants to utilize L- threonine as the sole source of carbon for growth.
last changed	2008/04/01 16:02