type	Journal Article
authors	Korpela, T. K.; Saarinen, R.
title	Affinity chromatography of B6-vitamin-dependent enzymes: purification of pig-heart branched-chain amino acid transaminase
journal	J Chromatogr
Activity	2.6.1.42
ui	85157974
year	(1985)
volume	318
number	2
pages	333-41.
keywords	Animal
abstract	Pig-heart branched-chain amino acid transaminase (EC 2.6.1.42) was purified to near homogeneity with a yield of 27%. A prepurification was performed by heat treatment, gel chromatography and DEAE-Sepharose methods. For the final step, several affinity gels were tested and the one containing cycloserine coupled to CNBr-activated Sepharose 4B was selected. This effected an additional five-fold purification with a yield of 60%. The present affinity results are compared with corresponding studies with other aminotransferases in an attempt to find possible universal techniques for their purification.
last changed	2002/11/12 16:17