type	Journal Article
authors	Gorin, F. A.; Mullinax, R. L.; Ignacio, P. C.; Neve, R. L.; Kurnit, D. M.
title	McArdle's & Hers' diseases: glycogen phosphorylase transcriptional expression in human tissues
journal	J Neurogenet
Activity	2.4.1.1
ui	88155137
year	(1987)
volume	4
number	6
pages	293-308.
keywords	Amino Acid Sequence
abstract	We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver(L) and muscle(M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-isozymes of phosphorylase in human fetal and adult tissues. The transcript encoding the M-isozyme is 3.4 kb; the L-isozyme transcript is 3.3 kb. Transcriptional expression of the L-isozyme in human and primate tissues was found to differ from the isozyme's reported tissue specificity in non-primate mammals. Furthermore, using degenerate oligonucleotide probes to two different coding regions of M-phosphorylase, a novel 4.1-kb transcript was demonstrated to be present in human fetal and adult brain.
last changed	2002/11/12 16:17