type	Journal Article
authors	Nishigaki, I.; Ichinose, H.; Tamai, K.; Nagatsu, T.
title	Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody
journal	Biochem J
Activity	4.1.1.28
ui	88326241
year	(1988)
volume	252
number	2
pages	331-5.
keywords	Adrenal Medulla/enzymology
abstract	Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr values of brain and adrenal-medulla enzyme were both estimated to be approx. 100,000 by gel- permeation chromatography. SDS/polyacrylamide-gel electrophoresis revealed a single band with an apparent Mr of 50,000. Western immunoblot analysis showed that the antibody recognized each enzyme. With regard to substrate specificity, pH-dependence and effect of pyridoxal 5'-phosphate as a cofactor, both enzymes were similar.
last changed	2002/11/12 16:17