type	Journal Article
authors	Yam, C. F.; Tunnicliff, G.; Ngo, T. T.; Barbeau, A.
title	A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain
journal	Comp Biochem Physiol B
Activity	4.1.1.15
ui	89250316
year	(1977)
volume	58
number	1
pages	115-7
keywords	Animal
abstract	1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations.
last changed	2002/11/12 16:17