type	Journal Article
authors	Ishikawa, T.; Okuno, E.; Kawai, J.; Kido, R.
title	Organ distribution, purification and characterization of kynureninase in Suncus murinus (Insectivora) and anthranilic acid level in the serum
journal	Comp Biochem Physiol B
Activity	3.7.1.3
ui	89324647
year	(1989)
volume	93
number	1
pages	107-11
keywords	Animal
abstract	1. The highest kynureninase activity was measured in Suncus liver compared with other organs tested. The holo-enzyme activity detected in liver was 25%. 2. The enzyme mainly localized in liver cytosol was purified to a single protein band by heat treatment, ammonium sulfate fractionation, DEAE-sepharose, hydroxyapatite and phenyl-sepharose column chromatography. 3. The purified enzyme was effective for both kynurenine and 3-hydroxykynurenine, and showed the optimum pH at 8.5 against both substrates. 4. Km values were 250 microM, 18 microM and 70 microM for kynurenine, 3-hydroxykynurenine and pyridoxal 5'-phosphate, respectively. Various compounds such as histidine, aspartate and nicotinamide enhanced the enzyme activities. 5. Anthranilate, the product of kynureninase, was present at a concentration in Suncus serum of 1.96 microM. This value was higher than those of rat and human.
last changed	2002/11/12 16:17