type	Journal Article
authors	Kaneko, M.; Kontani, Y.; Kikugawa, M.; Tamaki, N.
title	Inhibition of D-3-aminoisobutyrate-pyruvate aminotransferase by 5- fluorouracil and alpha-fluoro-beta-alanine
journal	Biochim Biophys Acta
Activity	2.6.1.40
ui	92338222
year	(1992)
volume	1122
number	1
pages	45-9.
keywords	Alanine Transaminase/antagonists & inhibitors
abstract	Among pyrimidine derivatives, we found that 5-fluorouracil potently inhibited purified rat liver D-3-aminoisobutyrate-pyruvate aminotransferase, whereas 5-fluorouridine did so to a much lesser extent. 5-Fluorouracil acted as a competitive inhibitor against beta- alanine with a Ki of 56 microM, and was uncompetitive against pyruvic acid, with a Ki of 73 microM. alpha-Fluoro-beta-alanine, a metabolite of 5-fluorouracil, was also a competitive inhibitor with respect to beta-alanine with a Ki of 8.0 mM. 5-Fluorouracil acted also as a competitive inhibitor of 4-aminobutyrate aminotransferase with respect to beta-alanine with a Ki value of 1.9 mM, and was uncompetitive against 2-oxoglutaric acid, with a Ki of 1.8 mM.
last changed	2002/11/12 16:17