type	Journal Article
authors	Petrarulo, M.; Pellegrino, S.; Marangella, M.; Cosseddu, D.; Linari, F.
title	High-performance liquid chromatographic microassay for L- glutamate:glyoxylate aminotransferase activity in human liver. Application in primary hyperoxaluria type 1
journal	Clin Chim Acta
Activity	2.6.1.4
ui	94139054
year	(1993)
volume	218
number	2
pages	193-200.
keywords	Chromatography, High Pressure Liquid/*methods
abstract	A rapid and sensitive liquid chromatographic technique to determine L- glutamate:glyoxylate and aminotransferase (EC 2.6.1.4) activity in human liver is described. Homogenised tissue was incubated for 60 min in the presence of substrates and the 2-oxoglutarate generated was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography. The procedure allowed the detection of the enzyme activity expressed by 7.5 micrograms of liver protein, it was more sensitive and less time- consuming than the spectrophotometric procedure previously used. No significant differences were found between normal controls and patients with primary hyperoxaluria. In an 8-month-infant with primary hyperoxaluria type 1, the enzyme activity was reduced to 16% of the average control values.
last changed	2002/11/12 16:17