Activities | Families | Sequences | Fold types | References | Help
B6db activities: 4.3.1.19

4.3.1.19
Description Threonine ammonia-lyase
Alternative names Threonine dehydratase;
L-threonine dehydratase;
Threonine dehydrase;
L-threonine deaminase;
L-threonine hydro-lyase (deaminating);
Threonine deaminase;
L-serine dehydratase;
Serine deaminase.
Catalyzed reaction L-threonine = 2-oxobutanoate + NH(3).
Cofactor Pyridoxal-phosphate.
Comments -!- The protein from P. putida does not require pyridoxal-phosphate.
-!- The enzyme from a number of sources also acts on L-serine, cf. EC 4.3.1.17.
-!- The reaction catalyzed probably involves initial elimination of water (hence the enzyme's original classification as EC 4.2.1.16), followed by isomerization and hydrolysis of the product with C-N bond breakage.
-!- Formerly EC 4.2.1.16.
Biodegradative threonine deaminase from Salmonella is dimeric, but apparently it becomes tetrameric (dimer of dimers) in the presence of the allosteric effectors AMP or CMP.
Prosite PROSITE; PDOC00149;
PDB 2GN1; 2GN2;
Organisms -Eubacteria -Plants -Fungi -Metazoa
 

Family 

4.3.1.19 (39)
 
Links Enzyme (activities) 4.3.1.19
BRENDA (activities) 4.3.1.19
KEGG (pathways) 4.3.1.19
PLPMDB (PLP mutants) 4.3.1.19
 
References
 Wu M, Crismaru CG, Salo O, Bovenberg RAL, Driessen AJ (2020) Impact of classical strain improvement of Penicillium rubens on amino acid metabolism during -lactam production Appl Environ Microbiol 86 e01561-19.

 Favrot L, Amorim Franco T, Blanchard JS. (2018) Biochemical Characterization of the Mycobacterium smegmatis Threonine Deaminase Biochemistry 57 6003-6012.

 Sharma R, Keshari D, Singh KS, Singh SK (2017) Biochemical and functional characterization of MRA_1571 of Mycobacterium tuberculosis H37Ra and effect of its down-regulation on survival in macrophages Biochem Biophys Res Commun 487 892-897.

 Husain A, Jeelani G, Sato D, Ali V, Nozaki T. (2010) Characterization of two isotypes of l-threonine dehydratase from Entamoeba histolytica. Mol Biochem Parasitol 170 100-4.

 Ohnishi M, Saito M, Wakabayashi S, Ishizuka M, Nishimura K, Nagata Y, Kasai S. (2008) Purification and characterization of serine racemase from a hyperthermophilic archaeon, Pyrobaculum islandicum J Bacteriol 190 1359-65.

 Risso C1, Van Dien SJ, Orloff A, Lovley DR, Coppi MV. (2008) Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens J Bacteriol 190 2266-74.

 Simanshu DK, Savithri HS, Murthy MR. (2006) Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation J Biol Chem 281 39630-41.

 Wessel PM, Graciet E, Douce R, Dumas R. (2000) Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments Biochemistry 39 15136-43.

 Wartmann, T.; Rosel, H.; Kunze, I.; Bode, R.; Kunze, G. (1998) AILV1 gene from the yeast Arxula adeninivorans LS3--a new selective transformation marker Yeast 14 1017-25.

 Bornaes, C.; Petersen, J.G.; Holmberg, S. (1992) Serine and threonine catabolism in Saccharomyces cerevisiae: the CHA1 polypeptide is homologous with other serine and threonine dehydratases Genetics 131 531-9.

 Mockel, B.; Eggeling, L.; Sahm, H. (1992) Functional and structural analyses of threonine dehydratase from Corynebacterium glutamicum J Bacteriol 174 8065-72.

 Eisenstein E (1991) Cloning, expression, purification, and characterization of biosynthetic threonine deaminase from Escherichia coli J Biol Chem 26 5801-7.

 Samach, A.; Hareven, D.; Gutfinger, T.; Ken-Dror, S.; Lifschitz, E. (1991) Biosynthetic threonine deaminase gene of tomato: isolation, structure, and upregulation in floral organs Proc Natl Acad Sci U S A 88 2678-82.

 Datta, P.;Goss, T.J.; Omnaas J.R.; Patil, R.V. (1987) Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases Proc Natl Acad Sci U S A 84 393-7.

Articles on 4.3.1.19
 
last changed 2019/06/20 13:01

B6db activities