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B6db activities:
Description Threonine ammonia-lyase.
Alternative names Threonine dehydratase;
L-threonine dehydratase;
Threonine dehydrase;
L-threonine deaminase;
L-threonine hydro-lyase (deaminating);
Threonine deaminase;
L-serine dehydratase;
Serine deaminase.
Catalyzed reaction L-threonine = 2-oxobutanoate + NH(3).
Cofactor Pyridoxal-phosphate.
Comments -!- The protein from P. putida does not require pyridoxal-phosphate.
-!- The enzyme from a number of sources also acts on L-serine, cf. EC
-!- The reaction catalyzed probably involves initial elimination of water (hence the enzyme's original classification as EC, followed by isomerization and hydrolysis of the product with C-N bond breakage.
-!- Formerly EC
Biodegradative threonine deaminase from Salmonella is dimeric, but apparently it becomes tetrameric (dimer of dimers) in the presence of the allosteric effectors AMP or CMP.
Prosite PROSITE; PDOC00149;
PDB 2GN1; 2GN2;
Organisms -Bacteria -Plants -Fungi -Metazoa

Family (37)
Links Enzyme (activities)
BRENDA (activities)
KEGG (pathways)
PLPMDB (PLP mutants)
 Husain A, Jeelani G, Sato D, Ali V, Nozaki T. (2010) Characterization of two isotypes of l-threonine dehydratase from Entamoeba histolytica. Mol Biochem Parasitol 170 100-4.

 Ohnishi M, Saito M, Wakabayashi S, Ishizuka M, Nishimura K, Nagata Y, Kasai S. (2008) Purification and characterization of serine racemase from a hyperthermophilic archaeon, Pyrobaculum islandicum. J Bacteriol 190 1359-65.

 Risso C1, Van Dien SJ, Orloff A, Lovley DR, Coppi MV. (2008) Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens J Bacteriol 190 2266-74.

 Simanshu DK, Savithri HS, Murthy MR. (2006) Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation J Biol Chem 281 39630-41.

 Wessel PM, Graciet E, Douce R, Dumas R. (2000) Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments Biochemistry 39 15136-43.

 Wartmann, T.; Rosel, H.; Kunze, I.; Bode, R.; Kunze, G. (1998) AILV1 gene from the yeast Arxula adeninivorans LS3--a new selective transformation marker Yeast 14 1017-25.

 Bornaes, C.; Petersen, J.G.; Holmberg, S. (1992) Serine and threonine catabolism in Saccharomyces cerevisiae: the CHA1 polypeptide is homologous with other serine and threonine dehydratases Genetics 131 531-9.

 Mockel, B.; Eggeling, L.; Sahm, H. (1992) Functional and structural analyses of threonine dehydratase from Corynebacterium glutamicum J Bacteriol 174 8065-72.

 Samach, A.; Hareven, D.; Gutfinger, T.; Ken-Dror, S.; Lifschitz, E. (1991) Biosynthetic threonine deaminase gene of tomato: isolation, structure, and upregulation in floral organs Proc Natl Acad Sci U S A 88 2678-82.

 Datta, P.;Goss, T.J.; Omnaas J.R.; Patil, R.V. (1987) Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases Proc Natl Acad Sci U S A 84 393-7.

Articles on
last changed 2009/06/29 13:02

B6db activities