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4.3.1.19 |
| Description |
Threonine ammonia-lyase. |
| Alternative names |
Threonine dehydratase.
L-threonine dehydratase.
Threonine dehydrase.
L-threonine deaminase.
L-threonine hydro-lyase (deaminating).
Threonine deaminase.
L-serine dehydratase.
Serine deaminase.
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| Catalyzed reaction |
L-threonine = 2-oxobutanoate + NH(3). |
| Cofactor |
Pyridoxal-phosphate. |
| Comments |
-!- The protein from P. putida does not require pyridoxal-phosphate.
-!- The enzyme from a number of sources also acts on L-serine, cf. EC 4.3.1.17.
-!- The reaction catalyzed probably involves initial elimination of water (hence the enzyme's original classification as EC 4.2.1.16), followed by isomerization and hydrolysis of the product with C-N bond breakage.
-!- Formerly EC 4.2.1.16. |
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Biodegradative threonine deaminase from Salmonella is dimeric, but apparently it becomes tetrameric (dimer of dimers) in the presence of the allosteric effectors AMP or CMP. |
| Prosite |
PROSITE; PDOC00149; |
| PDB |
2GN1; 2GN2; |
| Organisms |
-Bacteria -Plants -Fungi -Metazoa |
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Family |
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| Links |
Enzyme (activities) 4.3.1.19
BRENDA (activities) 4.3.1.19
KEGG (pathways) 4.3.1.19
PLPMDB (PLP mutants) 4.3.1.19
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| References |
Simanshu DK, Savithri HS, Murthy MR. (2006) Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation J Biol Chem 281 39630-41. Wessel PM, Graciet E, Douce R, Dumas R. (2000) Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments Biochemistry 39 15136-43. Wartmann, T.; Rosel, H.; Kunze, I.; Bode, R.; Kunze, G. (1998) AILV1 gene from the yeast Arxula adeninivorans LS3--a new selective transformation marker Yeast 14 1017-25.. Bornaes, C.; Petersen, J.G.; Holmberg, S. (1992) Serine and threonine catabolism in Saccharomyces cerevisiae: the CHA1 polypeptide is homologous with other serine and threonine dehydratases Genetics 131 531-9. . Godon, J.J.; Chopin, M.C.; Ehrlich, S.D. (1992) Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis J Bacteriol 174 6580-9.. Mockel, B.; Eggeling, L.; Sahm, H. (1992) Functional and structural analyses of threonine dehydratase from Corynebacterium glutamicum J Bacteriol 174 8065-72.. Samach, A.; Hareven, D.; Gutfinger, T.; Ken-Dror, S.; Lifschitz, E. (1991) Biosynthetic threonine deaminase gene of tomato: isolation, structure, and upregulation in floral organs Proc Natl Acad Sci U S A 88 2678-82.. Datta, P.;Goss, T.J.; Omnaas J.R.; Patil, R.V. (1987) Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases Proc Natl Acad Sci U S A 84 393-7.. |
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| last changed |
2008/04/01 16:41 |
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